Anti-Nuclear Matrix Protein p84 (THOC1) Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q96FV9

Core Product Specifications and Parameters

Biological Background: THOC1/p84 Function and Localization

• THOC1 couples mRNA transcription, processing, and nuclear export by associating with spliced mRNA and promoting NXF1-mediated export. The THOC1-2-3 core complex directly binds NXF1-NXT1 and stimulates DDX39B ATPase activity.

Related references: PMID:15833825 PMID:15998806 PMID:17190602 PMID:23222130 PMID:33191911

• THOC1 regulates transcriptional elongation of specific genes, contributing to gene expression control.

Related references: PMID:22144908

• It helps maintain genome stability by preventing co-transcriptional R-loop formation.
• Subcellularly, THOC1 localizes to nuclear speckles, nucleoplasm, nuclear matrix, and the cytoplasm.
• THOC1 participates in an apoptotic pathway involving caspase-6 activation, upregulation of BAK1/BCL2L1, and NF-kappa-B activation, independent of p53, and imposes a G2/M checkpoint; apoptosis is inhibited by RB1 binding.
• THOC1 is ubiquitously expressed, with very low levels in normal breast epithelium but high expression in breast tumors, correlating with aggressive phenotype, tumor size, and metastasis.
• Mutations in THOC1 are linked to autosomal dominant deafness DFNA86, possibly through hair cell dysfunction.
• The protein is subject to acetylation, phosphorylation, and ubiquitin-like modifications.

Experimental Guidance and Technical Tips

• The immunogen encompasses the C-terminal region (aa 428-657) of human THOC1, which may confer target specificity; consider confirming epitope recognition under your experimental conditions.
• This polyclonal antibody is suitable for WB, IHC-P, IF/ICC, and ELISA; titrating the antibody and optimizing protocol parameters is recommended for each assay.
• The antibody cross-reacts with mouse and rat THOC1, allowing multispecies studies; validate cross-reactivity in your specific models.
• Expected molecular weight is approximately 76 kDa; use appropriate molecular weight markers and verify the band in your sample type.
• For immunohistochemistry, consider antigen retrieval and use tissue sections known to overexpress THOC1 (e.g., breast carcinoma) as positive controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with one-stop access to IVD raw materials, supporting projects from concept to clinic. This anti-THOC1 (p84) rabbit polyclonal antibody is part of our growing catalog of antibodies for cancer and mRNA biology research. We supply validated antibody pairs, bulk reagents, and rare target sourcing to streamline your assay development pipeline.