Anti-Nuclear Matrix Protein p84 (THOC1) Monoclonal Antibody for WB, IP, ELISA - Q96FV9

Core Product Specifications and Parameters

Biological Background: Nuclear Matrix Protein p84 (THOC1) Function and Localization

• Core component of the THO subcomplex of the TREX complex, which couples mRNA transcription, processing, and nuclear export, specifically associating with spliced mRNA. Related references: PMID:15833825 PMID:15998806 PMID:17190602
• Required for efficient export of polyadenylated RNA, facilitating transport of mature mRNA from nucleus to cytoplasm. Related references: PMID:23222130
• The THOC1-THOC2-THOC3 core complex directly binds export factor NXF1-NXT1 and stimulates the ATPase activity of DDX39B/UAP56. Related references: PMID:33191911
• Regulates transcriptional elongation of a subset of genes. Related references: PMID:22144908
• Contributes to genome stability by preventing co-transcriptional R-loop formation.
• Participates in an apoptotic pathway involving caspase-6 activation, upregulation of BAK1/BCL2L1, and NF-kappa-B activation, independent of p53.
• Localizes to the nucleus (speckle, nucleoplasm, matrix) and cytoplasm, reflecting dynamic roles in mRNA metabolism.
• Ubiquitously expressed, with elevated levels in breast tumors and cancer cell lines, correlating with aggressive phenotypes.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 350–450 of human THOC1. This central region may allow detection of the full-length protein (~76 kDa) in denaturing western blots. When performing WB, consider using total cell lysates from human, mouse, or rat origin, as the antibody is reported to cross-react with all three species.
• For immunoprecipitation (IP) and ELISA, validate the antibody under your specific experimental conditions with proper positive and negative controls, including normal tissue controls for cancer-related studies.
• Given THOC1's roles in mRNA export and apoptosis, this antibody could be useful in studies on TREX complex dynamics, R-loop biology, and cancer progression. Confirm target specificity with siRNA knockdown or knockout models where possible.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines your diagnostic and research procurement by offering one-stop access to premium IVD raw materials. This anti-Nuclear Matrix Protein p84 (THOC1) monoclonal antibody is part of our extensive catalog supporting mRNA processing and apoptosis research. From validated antibody pairs to bulk ancillary reagents and rare target sourcing, CamelBio bridges your project from concept to clinic with reliable, cost-effective supply solutions.