Anti-NUCKS1 Rabbit Polyclonal Antibody for WB and ELISA - Q9H1E3

Core Product Specifications and Parameters

Biological Background: NUCKS1 Function and Localization

• NUCKS1 (Nuclear ubiquitous casein and cyclin-dependent kinase substrate 1), also known as P1, is encoded by the NUCKS1 gene in humans.
• It is a chromatin-associated protein involved in DNA repair by promoting homologous recombination (HR).
  Related references:
  PMID:26323318
• NUCKS1 binds to double-stranded DNA (dsDNA) and secondary DNA structures, such as D-loop structures, with lower affinity than RAD51AP1.
  Related references:
  PMID:26323318
• The protein localizes to the nucleus and associates with chromosomes.
• NUCKS1 is widely expressed, with highest levels in thyroid gland, prostate, uterus, and in fetal liver, thymus, and lung.
• It is classified as a phosphoprotein, suggesting regulation by phosphorylation.
• Alternative splicing of the NUCKS1 gene results in multiple transcript variants.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 100-200 of human NUCKS1 (NP_073568.2), which may provide specificity for this region.
• For Western blot analysis, consider using a standard protocol with a primary antibody dilution optimized for a 27 kDa target.
• For ELISA, validate the antibody against the peptide antigen with appropriate blocking and detection reagents.
• Cross-reactivity with mouse and rat has been demonstrated; when working with these species, verify the antibody performance in the relevant tissue or cell lysates.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents to support diagnostic development. With this NUCKS1 antibody, researchers and manufacturers can advance DNA repair and chromatin biology research, leveraging CamelBio's supply capabilities for rare target raw-material sourcing. Our integrated technical services and consulting help streamline your project from concept to clinical application.