Anti-NUCB2 Polyclonal Antibody for WB, ELISA - P80303

Core Product Specifications and Parameters

Biological Background: NUCB2 Function and Localization

• Nucleobindin-2 (NUCB2), also known as NEFA, HEL-S-109, gastric cancer antigen Zg4, and prepronesfatin, is a calcium-binding protein encoded by the NUCB2 gene in humans.
• It acts as a non‑receptor guanine nucleotide exchange factor (GEF) that binds to and activates the G protein alpha subunit GNAI3, implicating it in G protein signaling pathways (By similarity).
• NUCB2 plays a key role in calcium homeostasis and may regulate intracellular calcium levels (By similarity).
• The protein is processed into the anorexigenic peptide nesfatin‑1, which suppresses food intake and regulates energy homeostasis in a leptin‑independent manner. It also influences blood pressure through peripheral arterial resistance and, in intestinal epithelial cells, inhibits hepatic glucose production via the MC4R receptor, leading to increased cAMP and GLP‑1 secretion. Related references: PMID:39562740
• Subcellularly, NUCB2 localizes to the Golgi apparatus, endoplasmic reticulum, nuclear envelope, cytoplasm, and can be secreted; it also associates with membranes.
• Post‑translational modifications include phosphorylation, and the protein features a signal peptide, a DNA‑binding domain, and calcium‑binding repeats. Alternative splicing produces multiple isoforms.
• NUCB2 is predominantly expressed in the spleen, testis, and normal stomach.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 321–420 of human NUCB2. Consider epitope accessibility and any post‑translational modifications that might affect antibody binding in native versus denatured conditions.
• For Western blotting, a band around 50 kDa is expected. Optimize blocking and antibody dilution using a positive control lysate (e.g., spleen or testis tissue).
• For ELISA, validate coating antigen and detection conditions; the polyclonal nature may provide robust signal but requires careful titration.
• Cross‑reactivity with rat is reported; when using rat samples, perform appropriate controls to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories by providing one‑stop access to high‑quality IVD raw materials. For NUCB2‑related studies in energy metabolism, calcium signaling, or gastrointestinal research, we offer validated antibody pairs, monoclonal and polyclonal antibodies, and bulk ancillary reagents. Our sourcing capabilities extend to rare‑target raw materials, helping you streamline development from concept to clinic.