Anti-NUBP1 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P53384

Core Product Specifications and Parameters

Biological Background: NUBP1 Function and Localization

• NUBP1, also known as nucleotide-binding protein 1 (NBP1) or CIAO5, is a cytosolic Fe-S cluster assembly factor encoded by the NUBP1 gene in humans. Alternative splicing generates multiple isoforms.
• It is a key component of the cytosolic iron-sulfur (Fe/S) protein assembly (CIA) machinery, which is required for the maturation of extramitochondrial Fe-S proteins. NUBP1 forms a heterotetramer with NUBP2 that functions as a scaffold for de novo Fe-S cluster assembly and transfer to target apoproteins.
  Related references:
  PMID:18573874
• NUBP1 has been implicated in the regulation of centrosome duplication and negatively regulates cilium formation and structure, linking Fe-S cluster biogenesis to cell cycle and cytoskeletal organization.
• Subcellularly, NUBP1 is localized to the cytoplasm and nucleus, and it associates with various cytoskeletal structures, including the centrosome, centriole, basal body, cilium axoneme, and microtubule organizing center.
• The protein contains a 4Fe-4S cluster, an ATP-binding domain, and undergoes post-translational modifications such as acetylation and phosphorylation, reflecting its dynamic regulation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 201-320 of human NUBP1, suggesting that the antibody likely recognizes an internal epitope. Consider this when designing blocking peptides or alternative detection strategies.
• For Western blot (WB), expect a band at approximately 35 kDa (consistent with the predicted molecular weight of 34.5 kDa). Validate specificity using positive and negative control lysates.
• For immunohistochemistry (IHC-P), validate the antibody in relevant human, mouse, and rat tissue sections, considering the reported cross-reactivity. Optimize antigen retrieval and antibody dilution based on your specific experimental conditions.
• In ELISA applications, the antibody may be used for direct detection or as a capture reagent; empirical optimization of coating and detection conditions is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, core labs, and research institutes with seamless access to IVD raw materials, supported by technical consulting from concept to clinic. This anti-NUBP1 rabbit polyclonal antibody supports research in Fe-S cluster biogenesis, cytoskeleton biology, and related disease areas. Beyond this product, we supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents, and can assist with sourcing rare-target raw materials—offering a one-stop procurement experience tailored to your IVD development needs.