Anti-NUB1 Polyclonal Antibody for WB, IF/ICC, ELISA - Q9Y5A7

Core Product Specifications and Parameters

Biological Background: NUB1 Function and Localization

• NUB1 (NEDD8 ultimate buster 1), also designated Negative regulator of ubiquitin-like proteins 1 or Renal carcinoma antigen NY-REN-18, exists as two alternative splice isoforms.
• Serves as a specific down-regulator of the NEDD8 conjugation pathway by shuttling NEDD8, UBD, and their conjugates to the proteasome for degradation.
• Isoform 1 displays higher efficiency in promoting NEDD8 degradation compared to isoform 2.
• Predominantly localizes to the nucleus.
• Tissue expression is broad; isoform 1 is lowest in the pancreas, while isoform 2 shows minimal levels in leukocytes, liver, prostate, and skeletal muscle.
• Characterized by a coiled‑coil domain, repeat regions, and alternative splicing events.
• UniProt lists the calculated molecular weight as ~70.5 kDa; the antibody typically detects a band around 71 kDa.

Experimental Guidance and Technical Tips

• This rabbit polyclonal antibody was generated using a synthetic IFT140-derived peptide; confirm target recognition by NUB1-specific controls in your assays.
• For Western blotting, consider starting with a dilution range of 1:500–1:2,000 and optimize for your cell/tissue lysate.
• In immunofluorescence/immunocytochemistry, fixation and permeabilization conditions may influence nuclear staining intensity; test multiple protocols.
• ELISA can be used for screening and titer determination; include appropriate negative controls.
• Predicted cross-reactivity with human, mouse, and rat; verify reactivity in your species of interest if not listed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers, laboratories, and research teams with comprehensive IVD raw-material sourcing. This NUB1 polyclonal antibody complements our portfolio of validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and specialized procurement for rare targets—all designed to accelerate your projects in ubiquitin-like protein and proteasomal degradation research.