Anti-NUAK2 Polyclonal Antibody for IF/ICC - Q9H093

Core Product Specifications and Parameters

Biological Background: NUAK2 Function and Localization

• NUAK2 (NUAK family SNF1-like kinase 2), also known as SNARK or Omphalocele kinase 2, is a 69 kDa serine/threonine-protein kinase belonging to the AMPK-related kinase family.
• Acts as a stress-activated kinase that promotes tolerance to glucose starvation, enabling cellular survival under metabolic stress.
• Induces cell-cell detachment by facilitating F-actin to G-actin conversion, and promotes cell motility and invasiveness in tumor cells upon CD95/TNF-alpha stimulation via NF-kappa-B signaling.
• Protects cells from CD95-mediated apoptosis, suggesting a role in survival signaling.
• Phosphorylates LATS1 and LATS2, key kinases in the Hippo signaling pathway, thereby regulating downstream YAP1 nuclear localization.
• Critical for neural tube closure during embryonic development through LATS2 phosphorylation and subsequent YAP1 regulation. Related references: PMID:32845958
• Post-translationally modified by phosphorylation and acetylation, as indicated by proteomics identification and direct protein sequencing evidence.

Experimental Guidance and Technical Tips

• The immunogen used corresponds to the C-terminal region (amino acids 479-628) of human NUAK2, which is less conserved among related kinases. This design may enhance specificity; however, cross-reactivity should always be validated in your experimental system.
• For IF/ICC applications, optimize fixation and permeabilization conditions based on your cell type. Consider using human cell lines known to express NUAK2 (e.g., tumor cells with active CD95/TNF-alpha signaling) as positive controls.
• Due to confirmed human reactivity only, ensure that your sample species is compatible. If other species are needed, request a BLAST analysis or homology test in advance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a comprehensive sourcing platform for IVD raw materials. Our portfolio includes this anti-NUAK2 polyclonal antibody, along with validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets like signaling kinases. Partner with us to accelerate your projects in apoptosis, cell migration, and developmental biology research with reliable, one-stop supply solutions.