Anti-NUAK1 Rabbit Polyclonal Antibody for WB, ELISA - O60285

Core Product Specifications and Parameters

Biological Background: NUAK1 Function and Localization

• NUAK1, also known as ARK5 (AMPK-related protein kinase 5) or omphalocele kinase 1, is a serine/threonine-protein kinase belonging to the NUAK family of AMPK-related kinases.
• Controls cell adhesion by phosphorylating the myosin phosphatase target subunit PPP1R12A, which then binds 14-3-3 proteins, leading to decreased myosin light chain dephosphorylation and altered actomyosin contractility.
• Regulates cellular senescence and ploidy through phosphorylation of LATS1 at Ser-464, affecting its stability and consequently the Hippo signaling pathway.
• Participates in the DNA damage response by phosphorylating p53/TP53 at Ser-15 and Ser-392, and is recruited to the CDKN1A/WAF1 promoter to enhance p53-dependent transcription.
• Mediates UV radiation-induced DNA damage response: in complex with STK11, phosphorylates CDKN1A, promoting its degradation to enable efficient DNA repair. Related references: PMID:25329316
• Suppresses Fas-induced apoptosis by phosphorylating caspase-6 (CASP6), inhibiting its activation and subsequent cleavage of CFLAR.
• Localizes to both the nucleus and cytoplasm, consistent with its roles in transcription regulation and cytoskeletal dynamics.
• Highly expressed in heart and brain, with lower levels in skeletal muscle, kidney, ovary, placenta, lung, and liver; up-regulated in colorectal cancer cell lines, supporting its role in tumor progression.
• Undergoes post-translational modifications including acetylation, phosphorylation, and ubiquitin conjugation; alternative splicing generates multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 362–661, corresponding to the C-terminal region of human NUAK1; this region may contain regulatory domains, so consider antibody specificity in applications requiring N-terminal recognition.
• For western blotting, the predicted molecular weight is 74 kDa; use reducing conditions and validate with appropriate positive controls, such as colorectal cancer cell lines where NUAK1 is up-regulated.
• In ELISA, indirect detection may be performed using the recombinant immunogen as coating antigen; confirm cross-reactivity with rat samples in your assay system if needed.
• Always titrate the antibody in your specific experimental setup, as optimal dilutions can vary with sample type and detection method.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD manufacturers and researchers, providing validated antibody pairs, optimized polyclonal antibodies like this anti-NUAK1 antibody, and bulk ancillary reagents to accelerate assay development. Our portfolio supports key research areas such as oncology and cell signaling, and our team can assist with custom procurement of rare-target raw materials. Contact us to discuss your specific antibody or reagent needs for diagnostic and research projects.