Anti-NTSR1 Polyclonal Antibody for WB and ELISA - P30989

Core Product Specifications and Parameters

Biological Background: NTSR1 Function and Localization

• NTSR1, also known as neurotensin receptor type 1 or NTRH, is a high-affinity levocabastine-insensitive neurotensin receptor encoded by the NTSR1 gene.
• NTSR1 functions as a G protein-coupled receptor for the tridecapeptide neurotensin (NTS). Upon binding, it activates a phosphatidylinositol-calcium second messenger system, leading to downstream MAP kinase activation and protection against apoptosis. Related references: PMID:21725197 PMID:23140271 PMID:8381365
• It belongs to the G protein-coupled receptor (GPCR) family and possesses seven transmembrane helices, typical of this receptor class.
• The receptor is localized at the cell membrane and is enriched in membrane rafts, facilitating signaling compartmentalization.
• At the protein level, NTSR1 is expressed in the prostate, with lower detection in colon and peripheral blood mononuclear cells; brain expression is very low.
• Post-translational modifications include glycosylation and palmitoylation, and the receptor contains structural disulfide bonds.
• The protein has a calculated molecular weight of approximately 46 kDa (46259 Da), and its three-dimensional structure has been resolved.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal extracellular region (amino acids 1–70), which may favor detection of the cell surface receptor in native contexts.
• For Western blot, anticipate a band around 46 kDa; note that glycosylation may cause a slight upward shift in apparent molecular weight. Validate in relevant human and mouse tissue lysates, such as prostate or colon.
• In ELISA applications, the antibody can serve as capture or detection reagent; optimize coating and blocking conditions for best signal-to-noise ratio.
• Cross-reactivity with human and mouse is indicated; consider sequence alignment between species for experimental design.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, providing validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. For neurotensin receptor and GPCR research, we support your pipeline from concept to clinic with rare-target raw materials, including anti-NTSR1 antibodies, ensuring continuity and scalability.