Anti-NTS Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P30990

Core Product Specifications and Parameters

Biological Background: NTS Function and Localization

• The NTS gene encodes a precursor protein that is processed to yield the active peptides neurotensin and neuromedin N.
• Neurotensin functions as a signaling molecule with endocrine or paracrine actions, notably implicated in the regulation of fat metabolism.
• It also induces contraction of smooth muscle, highlighting its role in gastrointestinal and cardiovascular physiology.
• The protein is localized to secretory vesicles and is secreted into the extracellular space, consistent with its signaling role.
• Post-translational modifications include cleavage at pairs of basic residues to generate the mature peptides and formation of a pyrrolidone carboxylic acid at the N-terminus of neurotensin.
• Associated keywords include vasoactive, secreted, cytoplasmic vesicle, and signal peptide, underscoring its hormonal and vasoactive nature.

Experimental Guidance and Technical Tips

• This antibody was raised against a synthetic peptide spanning amino acids 100-170 of human NTS, a region within the propeptide sequence; consider its potential to recognize both precursor and cleavage products, and validate in relevant sample systems.
• For Western blotting, a band around 20 kDa is expected for the full-length precursor; under reducing conditions, detection of cleaved products may also occur.
• When performing immunohistochemistry (IHC-P), optimize antigen retrieval and antibody titration for tissue-specific expression patterns, particularly in brain, gut, and metabolic tissues.
• Cross-reactivity with mouse and rat orthologs has been observed; validate in your model system to confirm performance.
• For ELISA applications, use appropriate positive controls, such as recombinant NTS or known high-expressing tissue lysates.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and researchers with a comprehensive portfolio of IVD raw materials, including polyclonal antibodies like this NTS Rabbit pAb for neurobiology and metabolism research. We offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and tailored sourcing support for rare targets. Partner with us to streamline your procurement and accelerate your project from concept to clinic.