Anti-NTS Polyclonal Antibody for WB, IF-P, ELISA - P30990

Core Product Specifications and Parameters

Biological Background: Neurotensin/Neuromedin N Function and Localization

• Neurotensin/neuromedin N is a secreted peptide hormone encoded by the NTS gene, with a precursor molecular weight of ~20 kDa.
• It may function as an endocrine or paracrine factor in the regulation of fat metabolism and induces contraction of smooth muscle.
• The precursor protein is cleaved on a pair of basic residues to yield the active peptides neurotensin and neuromedin N.
• Subcellularly, it localizes to cytoplasmic secretory vesicles and is secreted upon stimulation.
• Keywords include vasoactive, indicating a role in modulating vascular tone, and pyrrolidone carboxylic acid, a post-translational modification.
• A 3D structure has been determined, and the protein has been identified in proteomics analyses.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 24-148 of human NTS, spanning both neurotensin and neuromedin N; validate specificity in your system, as it may detect precursor and processed forms.
• Recommended applications: Western blot (~20 kDa band), immunofluorescence for secretory vesicle staining, and ELISA; optimize conditions for each assay.
• Cross-reactivity with mouse and rat enables comparative studies; perform sequence alignment and tissue-specific validation.

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CamelBio provides IVD manufacturers and researchers with integrated access to high-quality raw materials like this anti-NTS polyclonal antibody, alongside validated antibody pairs, monoclonal options, and bulk ancillary reagents. Our sourcing expertise streamlines procurement for neuropeptide and metabolic research programs. Explore our catalog for comprehensive solutions tailored to your assay development needs.