Anti-NTPCR Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9BSD7

Core Product Specifications and Parameters

Biological Background: NTPCR Function and Localization

• NTPCR (Cancer-related nucleoside-triphosphatase), also known as THEP1, C1orf57, and HCR-NTPase, is a nucleotide phosphatase enzyme.
• It catalyzes the hydrolysis of nucleoside triphosphates (ATP, GTP, CTP, TTP, UTP) and, with lower efficiency, nucleoside diphosphates.
• The protein is classified as a hydrolase with ATP-binding and nucleotide-binding capabilities, suggesting a role in cellular nucleotide metabolism.
• According to UniProt keywords, NTPCR has a known 3D-structure and undergoes acetylation, a common post-translational modification.
• It is identified as a reference proteome component in humans, indicating its conserved functional importance.
• While detailed subcellular localization and tissue specificity are not yet fully characterized, its association with cancer (as implied by its name and some publications) makes it a target of interest in oncology research.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 91–190 of human NTPCR, a region that may provide target specificity; validate in the relevant sample system.
• For Western blot (WB), a band around 21 kDa is expected. Optimize primary antibody dilution empirically (e.g., 1:500–1:2000) and use appropriate loading controls.
• For immunofluorescence (IF/ICC), test fixation and permeabilization conditions; include a negative control (e.g., secondary antibody only) to assess non-specific binding.
• ELISA performance may require pairing with a detection antibody; consider checkerboard titrations to determine optimal concentrations.
• Because this is a polyclonal antibody, lot-to-lot consistency should be verified with a reference standard if used in quantitative assays.
• Cross-reactivity with mouse is reported, but cross-reactivity with other species has not been tested; confirm if your model organism is not human or mouse.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, labs, and research institutes streamlined access to premium IVD raw materials, supporting your development from concept to clinic. This anti-NTPCR rabbit polyclonal antibody exemplifies our capability to supply validated antibodies for cancer-related and nucleotide metabolism research. Beyond off-the-shelf products, we can source optimized monoclonal/polyclonal antibodies, validated antibody pairs for ELISA, and bulk ancillary reagents to meet your specific assay requirements.