Anti-NTMT1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9BV86

Core Product Specifications and Parameters

Biological Background: NTMT1 Function and Localization

• NTMT1, also known as NRMT, METTL11A, or NTM1A, is an N-terminal Xaa-Pro-Lys methyltransferase.
• Catalyzes mono-, di-, or tri-methylation of the N-terminal alpha-amino group of Ala, Gly, Ser, or Pro in the [A/G/S/P]-Pro-Lys motif after initiator Met removal.
• Essential for mitosis: methylates RCC1 to ensure normal bipolar spindle formation and chromosome segregation.
• Methylates multiple substrates, including CENPA (trimethylation of N-terminal Gly), RB1, SET, RPL23A, MYL2, MYL3, and KLHL31.
• Some substrates may require priming monomethylation by NTMT2 before full methylation by NTMT1 (PubMed:24090352). Related references: PMID:24090352
• Localizes to the nucleus, consistent with its role in nuclear processes.
• Uses S-adenosyl-L-methionine as a methyl donor; classified as a methyltransferase (transferring one-carbon groups).
• The gene undergoes alternative splicing, yielding multiple transcript variants.

Experimental Guidance and Technical Tips

• Because the antibody is a polyclonal raised against a full-length recombinant protein (aa 1-223), it recognizes multiple epitopes; consider this when optimizing dilutions for WB or IF/ICC.
• For Western blotting, a nuclear extract (e.g., HeLa cells) may serve as a positive control given the nuclear localization of NTMT1.
• Cross-reactivity with mouse and rat is reported; however, it is advisable to validate the antibody in the specific species and experimental system.
• In IF/ICC, anticipate nuclear staining; use appropriate fixation and permeabilization protocols to preserve nuclear architecture.
• For ELISA, the polyclonal nature can enhance sensitivity, but careful optimization of coating antigen and antibody concentration is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with convenient access to a wide array of IVD raw materials, including validated antibody pairs and optimized polyclonal antibodies like this NTMT1 antibody. For scientists delving into epigenetic regulation and mitotic processes, this product serves as a reliable tool for chromatin and cell cycle studies. From bulk ancillary reagents to rare target sourcing, our one-stop model supports your project from concept to clinic—please contact us to discuss your specific procurement needs.