Anti-NTF3 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P20783

Core Product Specifications and Parameters

Biological Background: NTF3 Function and Localization

• Neurotrophin-3 (NTF3, also known as NT-3, HDNF, NGF-2) is a secreted growth factor belonging to the neurotrophin family, which includes NGF, BDNF, and NT-4/5.
• It promotes the survival and differentiation of visceral and proprioceptive sensory neurons during development.
• NTF3 is expressed in both the brain and peripheral tissues, indicating roles beyond the nervous system.
• The protein is synthesized as a precursor that undergoes proteolytic cleavage at a pair of basic residues to release the mature active form.
• Mature NTF3 is a glycoprotein stabilized by disulfide bonds and contains a classical signal peptide for secretion.
• Alternative splicing generates multiple isoforms, and the protein has been characterized by 3D-structure analysis.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the nearly full-length mature human NTF3 (aa 19-257), covering most of the secreted protein; consider this when analyzing processed versus unprocessed forms.
• For Western blot, a band near 29 kDa is expected for the glycosylated mature monomer; validate in the relevant sample system and optimize lysate preparation and loading.
• IHC-P and ELISA have been reported as compatible applications; include appropriate controls and consider antigen retrieval for paraffin sections.
• Cross-reactivity with mouse suggests conserved epitopes; verify performance in your specific mouse model or tissue.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research groups with one-stop access to validated IVD raw materials, including this NTF3 antibody for neurotrophin signaling studies. Our supply capabilities span optimized polyclonal antibodies, recombinant proteins, and ancillary reagents, helping streamline your workflow from early-stage research to assay development. For targets like NTF3 or other rare neurotrophic factors, we also offer tailored sourcing and technical consultation to bridge critical raw material gaps.