Anti-NSUN5 Polyclonal Antibody for WB, IHC-P, ELISA - Q96P11

Core Product Specifications and Parameters

Biological Background: NSUN5 Function and Localization

• NSUN5 functions as an S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the C(5) position of cytosine 3782 (m5C3782) in 28S rRNA. Related references: PMID:23913415, PMID:31428936, PMID:31722427
• The m5C3782 modification catalyzed by NSUN5 enhances protein translation without affecting ribosome biogenesis or fidelity. Related references: PMID:31428936, PMID:31722427
• NSUN5 is required for corpus callosum and cerebral cortex development (by similarity).
• The protein localizes to the nucleolus within the nucleus.
• NSUN5 is ubiquitously expressed in human tissues, with prominent detection in placenta, heart, and skeletal muscle.
• It belongs to the NOL1/NOP2/Sun domain family and is associated with the Williams-Beuren syndrome chromosomal region; alternative splicing generates multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 127-429) encompasses a large central portion of NSUN5, potentially recognizing multiple isoforms and enabling detection in human, mouse, and rat due to conserved regions.
• For western blot, the predicted molecular weight is ~47 kDa; consider using whole-cell lysates from human, mouse, or rat cell lines or tissues as positive controls.
• IHC-P may require antigen retrieval and validation in relevant tissue sections; human placenta, heart, or skeletal muscle could serve as appropriate positive tissues based on expression profiles.
• ELISA performance can be evaluated using the recombinant immunogen as a standard, with careful optimization of coating and detection conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting. This NSUN5 antibody supports epigenetics and ribosome research, and CamelBio can supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to streamline your assay development. Contact us to leverage our integrated procurement solutions.