Anti-NSUN3 Rabbit Polyclonal Antibody for WB & ELISA - Q9H649

Core Product Specifications and Parameters

Biological Background: NSUN3 Function and Localization

• NSUN3 is a member of the NOL1/NOP2/Sun domain family of RNA methyltransferases and is also referred to as NOL1/NOP2/Sun domain family member 3.
• NSUN3 catalyzes the methylation of cytosine-34 in mitochondrial tRNA-Met to 5-methylcytosine (m5C), which is subsequently oxidized to 5-formylcytosine (f5c), enabling the anticodon to decode both AUG and AUA codons, thus expanding the codon recognition capacity of mitochondrial translation. Related references: PMID:27214402, PMID:27356879, PMID:27497299
• The protein is localized to the mitochondrial matrix, consistent with its role in decorating organellar tRNAs.
• Mutations in NSUN3 lead to combined oxidative phosphorylation deficiency 48 (COXPD48), a primary mitochondrial disease, underscoring its essential role in mitochondrial gene expression.
• As an S-adenosyl-L-methionine-dependent methyltransferase, NSUN3 specifically binds mitochondrial tRNA-Met and has been catalogued in large-scale proteomics efforts.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–120) of human NSUN3; consider this when designing blocking peptides or competition assays.
• Based on the predicted molecular weight (38 kDa), a band near 38 kDa is expected in western blotting; validate with appropriate positive controls (e.g., mitochondrial-enriched fractions from human, mouse, or rat cell lines).
• For ELISA, ensure the coating antigen preserves the immunogen epitopes; a direct ELISA with the recombinant protein may be used for titer determination.
• Cross-reactivity is reported for human, mouse, and rat; however, validate in your specific sample system as sequence homology in other regions may affect binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated one-stop partner for IVD raw-material sourcing, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For mitochondrial biology and rare metabolic disorders research, we provide seamless access to this NSUN3 antibody and support broader raw-material needs from concept to clinic. Explore tailored sourcing solutions with CamelBio for rare targets like NSUN3.