Anti-NSUN2 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q08J23

Core Product Specifications and Parameters

Biological Background: NSUN2 Function and Localization

• NSUN2, also known as MISU, MRT5, SAKI, or TRM4, is an RNA cytosine C(5)-methyltransferase that catalyzes the formation of 5-methylcytosine (m5C) in various RNA species, including tRNAs, mRNAs, and long non-coding RNAs. Related references: PMID:17071714 PMID:22995836 PMID:31199786
• It methylates specific cytosine residues in tRNA precursors: positions 34 and 48 of intron-containing tRNA(Leu)(CAA) and positions 48–50 of tRNA(Gly)(GCC), a modification essential for tRNA stability and generation of tRNA-derived fragments. Related references: PMID:17071714 PMID:22995836 PMID:31199786
• In mRNAs, NSUN2-mediated m5C methylation recruits the YBX1 protein, which binds to modified transcripts and protects them from decay, thereby enhancing mRNA stability. Related references: PMID:22395603 PMID:31358969 PMID:34556860
• m5C modifications in mRNA also facilitate nuclear export through recognition by the ALYREF/THOC4 adaptor protein, linking methylation to the nucleo-cytoplasmic trafficking of transcripts. Related references: PMID:28418038
• NSUN2 methylates non-coding vault RNAs (vtRNAs), such as VTRNA1.1, promoting their processing into small regulatory RNAs (svRNA4) that control epidermal differentiation. Related references: PMID:23871666 PMID:31186410
• It also targets mitochondrial tRNAs for C5-methylation, extending its regulatory role to mitochondrial gene expression. Related references: PMID:31276587
• Subcellularly, NSUN2 is found in the nucleolus, nucleoplasm, cytoplasm, mitochondria, and the mitotic spindle, and it is secreted via exosomes, reflecting its diverse functional compartments.
• Expressed in adult and fetal brain and in lymphoblastoid cells, NSUN2 is associated with key processes such as cell cycle, differentiation, and spermatogenesis; mutations are linked to intellectual disability.

Experimental Guidance and Technical Tips

• For Western blotting, consider loading 20–50 µg of total protein lysate; the endogenous NSUN2 band appears at approximately 86 kDa. Optimize blocking and primary antibody dilution for your sample.
• In immunohistochemistry (paraffin sections), heat-induced antigen retrieval (e.g., citrate buffer, pH 6.0) is recommended; titrate the antibody to achieve specific staining.
• For immunofluorescence, test fixation with 4% paraformaldehyde and permeabilization with 0.1% Triton X-100. Include appropriate controls to confirm localization.
• This antibody cross-reacts with mouse and rat NSUN2 according to supplier data; validate specificity in the species and tissue of interest using knock-out or knockdown models.
• The immunogen spans amino acids 617–708 (C-terminal region); be aware that the polyclonal serum may recognize multiple isoforms or degradation products.
• For ELISA applications, optimize the coating antigen concentration and antibody dilution, and include negative control wells to assess background.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports IVD manufacturers and research laboratories with a comprehensive portfolio of raw materials, including this anti-NSUN2 polyclonal antibody for applications in RNA epigenetics and cell differentiation studies. Our offering encompasses validated antibody pairs, recombinant proteins, and ancillary reagents, backed by a supply chain that accommodates everything from rare‑target discovery to high‑volume production. Partner with CamelBio to streamline your sourcing and accelerate the transition from concept to clinical application.