Anti-NSUN2 Monoclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - Q08J23

Core Product Specifications and Parameters

Biological Background: NSUN2 Function and Localization

• NSUN2 (also known as Misu, SAKI, TRM4) is an RNA cytosine C(5)-methyltransferase that catalyzes the formation of 5-methylcytosine (m5C) in tRNAs, mRNAs, and long non-coding RNAs, regulating RNA stability and processing. Related references: PMID:17071714, PMID:22995836, PMID:31199786
• It methylates specific cytosine residues in tRNA precursors, such as positions 34 and 48 of tRNA(Leu)(CAA) and positions 48, 49, and 50 of tRNA(Gly)(GCC), and is required for the generation of tRNA-derived fragments. Related references: PMID:17071714, PMID:22995836, PMID:31199786
• NSUN2 also mediates m5C modification of mitochondrial tRNAs. Related references: PMID:31276587
• It methylates mRNAs to enhance stability via YBX1 recognition and facilitate nucleocytoplasmic transport through ALYREF binding. Related references: PMID:22395603, PMID:31358969, PMID:34556860, PMID:28418038
• NSUN2 modifies non-coding RNAs, including vault RNAs (vtRNAs), promoting their processing into small regulatory RNAs involved in epidermal differentiation. Related references: PMID:23871666, PMID:31186410
• Apart from its methylation activity, NSUN2 is required for proper spindle assembly and chromosome segregation during mitosis. Related references: PMID:19596847
• NSUN2 localizes to the nucleus, nucleolus, cytoplasm, mitochondrion, spindle, and is secreted in extracellular exosomes.
• It is expressed in adult and fetal brain, lymphoblastoid cells, and is implicated in processes such as epidermal stem cell differentiation and spermatogenesis.
• Disease-associated variants in NSUN2 are linked to intellectual disability.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 617-708 of human NSUN2; consider verifying antibody specificity by testing with the recombinant immunogen or lysates from cells with known NSUN2 expression.
• For Western blot, a band around 86 kDa is expected; validate in human and mouse cell lysates known to express NSUN2 (e.g., lymphoblastoid cells or brain tissue).
• In immunohistochemistry and immunofluorescence, optimize antigen retrieval and fixation protocols; NSUN2’s nuclear and cytoplasmic localization may require careful permeabilization.
• For immunoprecipitation, confirm compatibility with downstream applications; note NSUN2’s potential association with RNA and protein complexes.
• Validate cross-reactivity in the intended species (human, mouse) using positive and negative control samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio stands as a reliable one-stop partner for IVD raw materials, technical services, and consulting, supporting your journey from concept to clinic. This NSUN2 Rabbit monoclonal antibody is part of a broad portfolio that includes validated antibody pairs, bulk ancillary reagents, and rare target sourcing solutions—enabling efficient development of RNA modification and methylation-based diagnostic assays. Whether you need scalable raw-material supply or specialized molecular biology support, CamelBio bridges your research to commercial success.