Anti-NSRP1 Polyclonal Antibody for WB, ELISA - Q9H0G5

Core Product Specifications and Parameters

Biological Background: NSRP1 Function and Localization

• NSRP1 (Nuclear speckle splicing regulatory protein 1), also known as CCDC55 or NSrp70, is an RNA-binding protein that plays a key role in pre-mRNA alternative splicing. Related references: PMID:21296756
• It mediates the regulation of alternative splicing events, influencing transcript diversity and protein isoform expression.
• Via interaction with CCDC118, NSRP1 may promote pre-adipocyte differentiation.
• NSRP1 localizes predominantly to the nucleus and is enriched in nuclear speckles, subnuclear domains associated with mRNA processing.
• Expressed in immune cells including dendritic cells, T-cells, B-cells, and natural killer cells, as well as secondary lymphoid organs like spleen and lymph nodes.
• Subject to post-translational modifications such as phosphorylation and ubiquitination, and contains coiled-coil domains.
• Mutations in NSRP1 have been linked to neurodevelopmental disorders, including epilepsy and intellectual disability.
• Keywords: mRNA processing, mRNA splicing, RNA-binding, nucleus, phosphoprotein, disease variant.

Experimental Guidance and Technical Tips

• For western blotting, NSRP1 has a predicted molecular weight of approximately 66 kDa. Use reducing conditions and load appropriate protein amounts (e.g., 20–50 µg lysate).
• Validate antibody specificity in cell lines or tissues known to express NSRP1, such as Jurkat (T-cell) or Raji (B-cell) lines, or lymphoid organs from mouse/rat.
• Consider using siRNA-mediated knockdown or CRISPR knockout controls to confirm band identity.
• For ELISA, determine the optimal coating concentration and detection antibody combinations empirically; this polyclonal antibody may serve as capture or detection reagent.
• Be aware of potential batch-to-batch variability inherent to polyclonal antibodies; include internal reference standards in each experiment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibodies, recombinant proteins, and ancillary reagents to streamline development workflows. This anti-NSRP1 polyclonal antibody supports splicing research and diagnostic assay development, and we can assist with bulk orders, custom conjugation, and complementary reagent procurement to meet your project needs. Whether you require optimized antibody pairs or rare-target reagents, CamelBio bridges the gap from concept to clinic.