Anti-NSMF Polyclonal Antibody for WB, ELISA - Q6X4W1

Core Product Specifications and Parameters

Biological Background: NSMF Function and Localization

• NSMF (also known as NELF or HH9) is a 60 kDa NMDA receptor synaptonuclear signaling and neuronal migration factor.
• It couples NMDA-sensitive glutamate receptor signaling to the nucleus, triggering long-lasting changes in dendritic and spine synapse architecture.
• NSMF participates in the cAMP response element-binding protein (CREB) shut-off signaling pathway.
• The protein stimulates outgrowth of olfactory axons and facilitates the migration of gonadotropin-releasing hormone (GnRH) and luteinizing-hormone-releasing hormone (LHRH) neurons.
• Subcellular localization includes nucleus, cytoplasm, cell membrane, dendrites, and postsynaptic densities.
• NSMF is highly expressed in adult and fetal brain, with lower levels in heart, liver, kidney, and other tissues.
• It is implicated in neurodevelopmental disorders such as hypogonadotropic hypogonadism and Kallmann syndrome, with alternative splicing and phosphorylation as key regulatory features.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a central region (aa 226-505) of human NSMF, which may allow recognition of multiple isoforms arising from alternative splicing; verify identity with appropriate controls.
• For Western blot, consider using brain tissue lysates given the high expression in brain; validate detection in relevant neuronal cell lines or mouse models.
• For ELISA, titrate the antibody against the recombinant immunogen or native protein in sample matrices of interest; optimize coating and detection conditions.
• Given the nuclear and synaptic localization, subcellular fractionation may enhance signal in WB when analyzing specific compartments.

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