Anti-NSL1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q96IY1

Core Product Specifications and Parameters

Biological Background: NSL1 Function and Localization

• NSL1 (also known as DC8, MIS14, and C1orf48) is the human homolog of the kinetochore-associated protein NSL1, encoded by the NSL1 gene.
• It is a core subunit of the MIS12 complex, which is indispensable for kinetochore assembly and stable microtubule attachment during mitosis.
• The MIS12 complex, including NSL1, mediates accurate chromosome alignment at the metaphase plate and faithful segregation into daughter cells.
• NSL1 predominantly localizes to the nucleus and concentrates at centromeric/kinetochore regions of chromosomes during cell division.
• As a phosphoprotein, NSL1 undergoes cell cycle–dependent phosphorylation, potentially modulating its interaction with other mitotic regulators.
• Alternative splicing of the NSL1 transcript generates multiple protein isoforms, suggesting functional diversity.
• Keywords from curated databases: 3D-structure, Alternative splicing, Cell cycle, Cell division, Centromere, Chromosome, Chromosome partition, Kinetochore, Mitosis, Nucleus, Phosphoprotein, Proteomics identification, Reference proteome.

Experimental Guidance and Technical Tips

• The immunogen spans residues 1–281 of full-length human NSL1 (NP_056286.3), covering the majority of the protein; the antibody is expected to recognize both major isoforms.
• For Western blot (WB), a predominant band around 32 kDa should be detected; validate with human mitotic cell lysates or cells synchronized in mitosis.
• For immunocytochemistry/immunofluorescence (ICC/IF), expect nuclear staining with distinct kinetochore foci in mitotic cells; co-stain with a centromere marker (e.g., CENP-A) to confirm specificity.
• Cross-reactivity with other species has not been tested; confirm if required for non-human models.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio acts as your dedicated one-stop sourcing bridge, connecting diagnostic developers and research laboratories with high-quality IVD raw materials. This anti-NSL1 polyclonal antibody supports fundamental mitotic and chromosome biology studies, and CamelBio can provide matched antibody pairs, optimized monoclonal or polyclonal reagents, and bulk ancillary biochemicals to accelerate your workflow. For scarce or customized targets, inquire about our flexible sourcing and supply-chain services.