Anti-NSF Rabbit Polyclonal Antibody for WB, IF/ICC, IP, ELISA - P46459

Core Product Specifications and Parameters

Biological Background: NSF Function and Localization

• Protein Identity: NSF (N-ethylmaleimide-sensitive fusion protein), also known as Vesicle-fusing ATPase, SKD2, or SEC18, is a member of the AAA ATPase family encoded by the NSF gene (Gene ID: 4905).
• Core Function: This protein is essential for vesicle-mediated transport, catalyzing the fusion of transport vesicles within the Golgi cisternae and facilitating transport from the endoplasmic reticulum to the Golgi stack.
• Cargo Delivery: NSF appears to act as a fusion protein required for delivering cargo proteins to all Golgi compartments regardless of vesicle origin, ensuring proper intracellular trafficking.
• AMPAR Interaction: NSF interacts with the AMPA receptor subunit GRIA2, influencing its membrane cycling and thereby modulating synaptic plasticity (By similarity).
• Subcellular Localization: The protein predominantly resides in the cytoplasm, consistent with its role in membrane fusion events.
• Key Properties and Disease Associations: NSF exhibits ATP-binding and hydrolase activity, requires magnesium as a cofactor, and undergoes phosphorylation and acetylation. Mutations in NSF are linked to developmental and epileptic encephalopathy (DEE96) and intellectual disability.

Experimental Guidance and Technical Tips

• Immunogen Design: The antibody was raised against a recombinant fragment spanning amino acids 251-400 of human NSF, a region within the D2 ATPase domain. This provides specificity for the native protein.
• Western Blotting: For WB, a band around 83 kDa corresponding to endogenous NSF can be expected. Optimize blocking and detection conditions for your sample type (human, mouse, or rat lysates).
• Immunofluorescence/Immunocytochemistry: In IF/ICC, NSF should show cytoplasmic staining. Permeabilization is required; use appropriate fixation methods to preserve epitopes.
• Immunoprecipitation: The polyclonal nature allows efficient capture of native NSF complexes. Validate the IP efficiency in your system and consider cross-linking if needed.
• ELISA: The antibody can be used as a capture or detection reagent; perform checkerboard titrations to determine optimal coating and detection concentrations.
• General Considerations: As a rabbit polyclonal, it recognizes multiple epitopes, reducing the risk of signal loss due to single epitope masking. Always include positive and negative controls specific to your experimental conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a strategic sourcing partner for IVD raw materials, providing one-stop access to validated antibodies, antigen pairs, and ancillary reagents for NSF and related trafficking proteins. Our supply chain supports diagnostic assay development from concept to clinic, including bulk polyclonal antibodies and customized solutions. Reach out to explore how we can streamline your NSF-targeted immunodetection workflows.