Anti-NSDHL Monoclonal Antibody for WB, ELISA - Q15738

Core Product Specifications and Parameters

Biological Background: NSDHL Function and Localization

• NSDHL (sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating), also known as Protein H105e3, is a 41.9 kDa enzyme encoded by the NSDHL gene (GeneID: 50814) on the X chromosome.
• It catalyzes the NAD(P)(+)-dependent oxidative decarboxylation of the C4 methyl groups of 4-alpha-carboxysterols, an essential step in post-squalene cholesterol biosynthesis.
• Subcellularly, NSDHL localizes primarily to the endoplasmic reticulum membrane and lipid droplets, consistent with its role in sterol and lipid metabolism.
• The enzyme is also reported to play a role in the regulation of endocytic trafficking of the epidermal growth factor receptor (EGFR).
• NSDHL is a multi-pass membrane protein with a predicted transmembrane helix; it belongs to the sterol biosynthesis oxidoreductase family and requires NAD as a cofactor.
• Expression of NSDHL is detected in multiple tissues, including brain, heart, liver, lung, kidney, skin, and placenta.
• Mutations in NSDHL are associated with X-linked chondrodysplasia punctata 2 (CDPX2), a disorder characterized by ichthyosis and intellectual disability, highlighting its clinical relevance in human disease.

Experimental Guidance and Technical Tips

• For western blotting, a band corresponding to NSDHL is expected at approximately 42 kDa; consider using total cell or tissue lysates from human sources known to express NSDHL (e.g., liver, skin).
• The immunogen covers amino acids 50–150 of human NSDHL; verify that the epitope is accessible under denaturing and reducing conditions for WB.
• For ELISA, use recombinant NSDHL protein or peptide as a standard to establish assay conditions; pairing with a detection antibody may require optimization.
• As a rabbit monoclonal antibody, it typically offers high specificity and lot-to-lot consistency; however, each new application should be validated in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with one-stop access to IVD raw materials, technical services, and consulting—from concept to clinic. This NSDHL antibody, targeting a key enzyme in cholesterol biosynthesis, is well-suited for research into metabolic disorders, skin barrier dysfunction, and EGFR trafficking. In addition to validated antibody pairs and bulk reagents, CamelBio supports rare target raw-material sourcing to accelerate your assay development and manufacturing needs.