Anti-NSD3/WHSC1L1 Polyclonal Antibody for WB, ELISA - Q9BZ95

Core Product Specifications and Parameters

Biological Background: NSD3/WHSC1L1 Function and Localization

• Histone-lysine N-methyltransferase NSD3 (also known as WHSC1L1, KMT3F, KMT3G, WHISTLE, pp14328) is a member of the NSD family of SET-domain-containing methyltransferases.
• Preferentially catalyzes dimethylation of histone H3 at lysine 4 (H3K4me2) and lysine 27 (H3K27me2), thereby regulating transcriptional activation (via H3K4me2) and repression (via H3K27me2).
• Localizes to the nucleus and associates with chromatin, consistent with its role in epigenetic transcriptional regulation.
• Exhibits high expression in brain, heart, and skeletal muscle, with lower levels in liver and lung, suggesting tissue-specific epigenetic modulation.
• Contains a SET domain, multiple PHD zinc-finger domains, and a PWWP domain, contributing to chromatin binding and methyltransferase activity.
• Functionally implicated in chromosomal rearrangements and oncogenesis, categorized as a proto-oncogene.
• Undergoes alternative splicing and post-translational modifications including acetylation, phosphorylation, and ubiquitination, which may modulate its activity and stability.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 430–590, which likely includes conserved regions of the SET domain; this polyclonal antibody may recognize multiple epitopes and work across human and mouse samples.
• For Western blotting, the predicted molecular weight is approximately 162 kDa; consider using a high–molecular-weight gel and appropriate transfer conditions. Validate the antibody in your model system, as alternative splicing and post-translational modifications may yield additional bands.
• This antibody is applicable for ELISA; when setting up the assay, consider using recombinant NSD3 protein or cell lysates from tissues with high expression (e.g., brain, heart) to optimize detection.
• Given the nuclear localization, nuclear extraction protocols may enrich the target signal in WB.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic developers and researchers with one-stop access to high-quality IVD raw materials, including validated antibody pairs and polyclonal antibodies like this NSD3/WHSC1L1 antibody for epigenetic studies. Our supply portfolio covers bulk ancillary reagents and rare target sourcing to streamline your assay development from concept to clinic.