Anti-NSD1 Polyclonal Antibody for WB and ELISA - Q96L73

Core Product Specifications and Parameters

Biological Background: NSD1 Function and Localization

• NSD1 (nuclear receptor-binding SET domain-containing protein 1) is a histone-lysine N-methyltransferase that specifically dimethylates lysine 36 of histone H3 (H3K36me2), a mark associated with transcriptional elongation.
• It functions as a transcriptional intermediary factor capable of both repressing and activating transcription, depending on the cellular context.
• The protein contains multiple chromatin-related domains, including a SET domain, PHD fingers, and zinc-finger motifs, characteristic of chromatin regulatory methyltransferases.
• Mutations in NSD1 are linked to Sotos syndrome, an overgrowth disorder, highlighting its disease relevance (alias SOTOS1).
• Subcellularly, NSD1 localizes to the nucleus and associates with chromosomes, consistent with its role in epigenetic regulation.
• It is expressed in various tissues, notably fetal and adult brain, kidney, skeletal muscle, spleen, and thymus.

Experimental Guidance and Technical Tips

• For Western blot, note the predicted molecular weight of approximately 297 kDa; consider using low-percentage gels and extended transfer times for high molecular weight proteins.
• The immunogen corresponds to the N-terminal region (aa 60-260) of human NSD1; assess specificity by using appropriate controls.
• Validate cross-reactivity with mouse samples due to sequence homology.
• ELISA optimization may require testing different coating conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in providing comprehensive IVD raw material solutions, offering validated antibodies like this NSD1 polyclonal antibody to support epigenetic and oncology research. Our platform streamlines sourcing of high-quality antibodies, paired detection reagents, and customized rare-target raw materials, enabling diagnostic developers from concept to clinic. Contact us to explore bulk orders, alternative formats, or custom antibody development services.