Anti-NSA2 Rabbit Polyclonal Antibody for WB and ELISA - O95478

Core Product Specifications and Parameters

Biological Background: NSA2 Function and Localization

• NSA2, also designated as Hairy cell leukemia protein 1 (HCLG1) or TGF-beta-inducible nuclear protein 1 (TINP1), is a conserved nucleolar protein essential for ribosome biogenesis.
• It participates in the maturation of the 60S ribosomal subunit and is implicated in quality control of pre-60S particles (By similarity).
• NSA2 is localized to the nucleolus, the subnuclear compartment where rRNA transcription, processing, and ribosomal subunit assembly occur.
• Keywords associated with NSA2 include ribonucleoprotein, ribosome biogenesis, and rRNA processing, underscoring its role in ribosomal assembly.
• The protein is subject to post-translational modifications, including phosphorylation and ubiquitin-like conjugation (isopeptide bond), which may regulate its function.
• The alternative names HCLG1 and TINP1 originate from early studies, though the protein's primary role is in ribosome maturation.

Experimental Guidance and Technical Tips

• The immunogen comprises a recombinant fusion protein covering amino acids 1-260 of human NSA2. This full-length antigen ensures recognition of multiple epitopes, enhancing sensitivity in detection.
• For Western blot (WB), whole cell lysates from human, mouse, or rat cell lines are suitable. A band at approximately 30 kDa is expected.
• For ELISA, the antibody can be used as a coating or detection antibody; optimal working concentrations should be determined experimentally.
• As a rabbit polyclonal antibody, lot-to-lot consistency may vary; we recommend validating each new batch in the intended assay system.
• Standard blocking and washing conditions for WB (e.g., 5% non-fat milk in TBST) are generally compatible, but individual optimization is advised.

CamelBio: Your One-Stop Sourcing Bridge

At CamelBio, we empower diagnostic manufacturers, labs, and research institutes with a comprehensive portfolio of IVD raw materials and technical solutions—from concept to clinic. This NSA2 rabbit polyclonal antibody exemplifies our offering for ribosomal biogenesis and cancer marker research. Beyond antibodies, we provide validated antibody pairs, optimized recombinant proteins, bulk ancillary reagents, and custom sourcing for rare targets, all backed by expert technical consultation. Let CamelBio be your streamlined partner for reliable raw-material supply.