Anti-NRXN3 Polyclonal Antibody for WB, ELISA - Q9HDB5/Q9Y4C0

Core Product Specifications and Parameters

Biological Background: NRXN3 Function and Localization

• Neurexin-3-beta (NRXN3) is a neuronal cell surface protein encoded by the NRXN3 gene, belonging to the neurexin family of synaptic adhesion molecules.
• Functions in cell recognition and adhesion, and participates in trans-synaptic signaling by binding to cerebellins and postsynaptic GRID1, modulating NMDA and AMPA receptor activity at hippocampal synapses (By similarity).
• Localizes specifically to the presynaptic cell membrane, consistent with its role in synaptic organization and neurotransmitter receptor regulation.
• Expressed in blood vessel walls at the protein level, suggesting potential involvement in angiogenesis-related processes.
• NRXN3 undergoes extensive alternative promoter usage and splicing, producing multiple isoforms that contribute to synaptic diversity.
• Contains characteristic structural domains including a signal peptide, transmembrane helix, and LNS (laminin/neurexin/sex hormone-binding globulin) repeats; also features glycosylation and heparan sulfate modification sites, classifying it as a proteoglycan.
• Binds calcium and other metal ions, which may influence its adhesion properties and synaptic complex formation.
• Keywords compiled from UniProt highlight its roles in cell adhesion, synapse, alternative splicing, angiogenesis, and proteoglycan biology.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a region within human NRXN3 (amino acids 373-432). Consider verifying reactivity with the specific isoform expressed in your sample system, as multiple isoforms exist.
• Suitable for Western blotting (WB) and ELISA. For WB, multiple bands may be observed due to various isoforms (predicted molecular weights range from 43 kDa to 180 kDa). Confirm band specificity with appropriate controls.
• Reported cross-reactivity with human, mouse, and rat. If using in other species, validate cross-reactivity experimentally before use.
• For optimal results, we recommend optimizing antibody dilutions and incubation conditions in your specific laboratory setup.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive portfolio of IVD raw materials, including this NRXN3 antibody, to support your research in synaptic biology and cell adhesion. As your one-stop sourcing bridge, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing tailored to your diagnostic development needs from concept to clinic.