Anti-NRXN1 Rabbit Polyclonal Antibody for WB, ELISA - P58400 / Q9ULB1

Core Product Specifications and Parameters

Biological Background: NRXN1 Function and Localization

• NRXN1 encodes neurexin-1-beta, a neuronal cell surface protein that belongs to the neurexin family of synaptic adhesion molecules.
• It functions in cell recognition and cell adhesion by forming trans-synaptic complexes with postsynaptic neuroligins, cerebellins, and GRID1 (glutamate receptor delta-1).
• This protein plays a critical role in formation and maturation of synaptic junctions and regulation of NMDA and AMPA receptor activity at hippocampal synapses.
• Neurexin-1-beta is localized to the presynaptic cell membrane and is enriched at presynaptic terminals.
• Multiple isoforms are generated by alternative promoter usage and alternative splicing, resulting in protein weight variants (15kDa, 46kDa, 161kDa, 164kDa, 169kDa) as indicated by product data.
• Post-translational modifications include glycosylation, heparan sulfate proteoglycan modification, and phosphorylation; it also contains calcium-binding EGF-like domains.
• Keywords: 3D-structure, Alternative splicing, Angiogenesis, Calcium, Cell adhesion, Cell membrane, Cell projection, Glycoprotein, Heparan sulfate, Membrane, Metal-binding, Phosphoprotein, Proteoglycan, Signal, Synapse, Transmembrane helix.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular region (aa 31-310) of human NRXN1, which is conserved in mouse, supporting cross-reactivity.
• Consider using the antibody for WB detection of NRXN1 isoforms; note the multiple predicted molecular weights due to alternative splicing. Optimize blocking and antibody dilution conditions for each application.
• For ELISA, validate in the relevant sample system; polyclonal antibodies may require careful titration due to batch variability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers with one-stop access to IVD raw materials, including validated antibody pairs and bulk reagents for synaptic biology targets like NRXN1. Our sourcing network can efficiently fulfill requirements for rare or niche raw materials, streamlining your assay development from concept to clinic. Contact us to explore tailored supply solutions for your next project.