Anti-NRIP1 Polyclonal Antibody for WB, ELISA - P48552

Core Product Specifications and Parameters

Biological Background: NRIP1 Function and Localization

• NRIP1 (Nuclear receptor-interacting protein 1), also known as RIP140 (Receptor-interacting protein 140), is a transcriptional coregulator that modulates activation by steroid receptors such as NR3C1 (glucocorticoid receptor), NR3C2 (mineralocorticoid receptor), and ESR1 (estrogen receptor). It also mediates transcriptional repression by nuclear hormone receptors.
• NRIP1 acts as a positive regulator of circadian clock gene expression by coactivating RORA and RORC to stimulate transcription of BMAL1, CLOCK, and CRY1, thereby contributing to the maintenance of biological rhythms.
• This protein has been implicated in renal development, supported by evidence linking it to congenital anomalies of the kidney and urinary tract (CAKUT3). Related references: PMID:28381549
• NRIP1 is involved in the regulation of ovarian function, as indicated by similarity data.
• Subcellularly, NRIP1 localizes to the nucleus, consistent with its role in transcriptional regulation.
• Post-translational modifications of NRIP1 include acetylation, phosphorylation, and ubiquitin-like (Ubl) conjugation (e.g., isopeptide bond formation), which likely modulate its activity and stability.
• The protein has a predicted molecular weight of ~127 kDa (126,942 Da) and contains repeat regions characteristic of transcriptional coregulators.

Experimental Guidance and Technical Tips

• For Western blot, consider using positive control lysates from human or mouse tissues known to express NRIP1, such as kidney or reproductive organs, to validate band specificity.
• The immunogen encompasses the C-terminal region (aa 939–1158); thus, the antibody may recognize full-length NRIP1 and potential C-terminal fragments.
• When performing ELISA, direct coating of the recombinant immunogen or capture of native NRIP1 from cell lysates can be explored; appropriate blocking and washing steps are essential.
• Cross-reactivity with human and mouse NRIP1 is reported; however, validation in the specific experimental system (e.g., cell line or tissue type) is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with one-stop access to high-quality IVD raw materials, including this NRIP1 polyclonal antibody for transcriptional regulation and developmental biology studies. We support your workflow from concept to clinic with validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and customized sourcing for rare targets.