Anti-NRG4 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q8WWG1

Core Product Specifications and Parameters

Biological Background: NRG4 Function and Localization

• NRG4 (Neuregulin-4) is also known as HRG4. The UniProt-described isoform is the pro-neuregulin-4, membrane-bound isoform, which can be proteolytically processed to release a soluble growth factor.
• It functions as a low-affinity ligand for the ERBB4 tyrosine kinase receptor. Upon binding, it concomitantly recruits ERBB1 and ERBB2 coreceptors, leading to ligand-stimulated tyrosine phosphorylation and activation of ERBB receptors. It does not bind ERBB1, ERBB2, or ERBB3 alone.
• Belongs to the neuregulin family of EGF-like growth factors, which are critical for cell signaling in development and cancer.
• Subcellular localization: cell membrane (as a transmembrane protein) and secreted (after cleavage). The full-length 13 kDa pro-form is membrane-anchored, while the soluble ectodomain acts as a paracrine signal.
• Key structural features include an EGF-like domain, disulfide bonds, and glycosylation sites. The EGF-like domain is essential for receptor binding.
• Relevant keywords: Cell membrane, Secreted, Disulfide bond, EGF-like domain, Glycoprotein, Growth factor, Transmembrane, Transmembrane helix.

Experimental Guidance and Technical Tips

• The immunogen was a synthetic peptide from the N-terminal region (amino acids 1-100) of human NRG4. This region likely contains the signal peptide and part of the EGF-like domain, suggesting the antibody may recognize both the full-length pro-form and the cleaved mature ligand.
• For Western blot (WB), the predicted molecular weight of the pro-protein is approximately 13 kDa, but post-translational modifications and cleavage may yield additional bands; consider using appropriate positive controls (e.g., recombinant NRG4 or cell lines expressing NRG4).
• In immunohistochemistry (IHC-P), membrane and secreted localizations should be expected; validate staining patterns in tissues known to express ERBB4, such as neural and epithelial tissues.
• ELISA performance suggests applicability for quantitative detection of NRG4 in serum, plasma, or cell culture supernatants; however, assay conditions need optimization for each sample type.
• Cross-reactivity with mouse and rat indicates that the epitope is conserved; verify sequence alignment if using non-human samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a comprehensive IVD raw-material sourcing partner supporting diagnostic manufacturers from early development to clinical stages. This anti-NRG4 polyclonal antibody supports research into ERBB signaling and growth factor biology, offering a reliable reagent for assay development and target validation. CamelBio offers validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and specialized sourcing for rare targets, streamlining your supply chain for IVD projects.