Anti-NRG1 Polyclonal Antibody for WB, ELISA - Q02297

Core Product Specifications and Parameters

Biological Background: NRG1 Function and Localization

• Pro-neuregulin-1 (NRG1) is a membrane-bound growth factor and direct ligand for the ERBB3 and ERBB4 tyrosine kinase receptors, encoded by the NRG1 gene (also known as GGF, HGL, HRGA, NDF, SMDF).
• Ligand engagement triggers ERBB receptor activation and tyrosine phosphorylation, recruiting coreceptors ERBB1 and ERBB2. Related references: PMID:10867024 PMID:7902537
• NRG1 binds to integrins (ITGAV:ITGB3 or ITGA6:ITGB4) via its EGF domain, forming a ternary complex with ERBB3 that is essential for downstream signaling. Related references: PMID:20682778
• Multiple NRG1 isoforms (types I–IV) arise from alternative splicing, with distinct tissue distributions: type I in endocardium, isoform 6 as the major neuronal form, isoform 9 in skeletal muscle, and isoform 10 in sensory and motor neurons.
• Subcellular localization includes cell membrane, secreted forms, and nucleus, reflecting its roles in juxtacrine, paracrine, and intracrine signaling.
• Key structural features include an EGF-like domain, immunoglobulin domain, transmembrane helix, and conserved disulfide bonds; NRG1 is classified as a glycoprotein and growth factor.
• NRG1 regulates diverse processes: growth/differentiation of epithelial, glial, neuronal, and skeletal muscle cells; Schwann cell proliferation; cardiac trabeculation; mammary gland development; and neuromuscular junction formation.
• Ligand-dependent ERBB4 endocytosis is critical for NRG1-activated MAPK/ERK and AKT1 signaling in neurons (By similarity).

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (amino acids 1–210), which may detect multiple isoforms; verify specificity in your target tissue or cell line.
• For Western blot (WB), consider the predicted molecular weight of ~70 kDa, but note that isoform-specific bands may vary; use appropriate positive controls such as human brain or breast tissue lysates.
• In ELISA applications, direct and sandwich formats are possible; pair with antibodies targeting distinct epitopes if developing a quantitative assay.
• Validate reactivity in human, mouse, and rat samples, as cross-reactivity is indicated; include species-specific positive controls to confirm performance.
• Given NRG1’s role in ERBB signaling, consider functional assays to correlate expression with pathway activation (e.g., phospho-ERBB4 or AKT).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers, labs, and research institutes with a seamless supply chain for IVD raw materials, from monoclonal/polyclonal antibodies to ancillary reagents. For NRG1-related research in neurodevelopment, oncology, or cardiac biology, we provide validated antibody pairs, bulk reagents, and rare target sourcing to accelerate your concept-to-clinic journey. Partner with CamelBio to streamline your IVD development with reliable, high-quality raw materials.