Anti-NRF2 Polyclonal Antibody for WB, IHC-P, ELISA - Q16236

Core Product Specifications and Parameters

Biological Background: NRF2 Function and Localization

• NRF2 is a transcription factor that plays a key role in the response to oxidative stress by binding to antioxidant response (ARE) elements in the promoter region of cytoprotective genes, such as phase 2 detoxifying enzymes, and promoting their expression (PubMed:11035812, PubMed:19489739, PubMed:29018201).
  Related references: PMID:11035812, PMID:19489739, PMID:29018201
• In normal conditions, NRF2 is ubiquitinated and degraded in the cytoplasm by the BCR(KEAP1) complex (PubMed:11035812, PubMed:15601839, PubMed:29018201).
  Related references: PMID:11035812, PMID:15601839, PMID:29018201
• Upon oxidative stress, electrophile metabolites inhibit the KEAP1 complex, allowing NRF2 nuclear accumulation, heterodimerization with small Maf proteins, and transcriptional activation of target genes (PubMed:19489739, PubMed:29590092).
  Related references: PMID:19489739, PMID:29590092
• Selective autophagy also activates the NRF2 pathway via KEAP1-SQSTM1/p62 interaction, promoting cytoprotective gene expression (PubMed:20452972).
  Related references: PMID:20452972
• NRF2 represses antiviral cytosolic DNA sensing by suppressing STING1 expression and inhibits replication of several pathogenic viruses, including SARS-CoV-2, through an interferon-independent mechanism (PubMed:30158636, PubMed:33009401).
  Related references: PMID:30158636, PMID:33009401
• Subcellular localization includes cytoplasm, cytosol, and nucleus. It is widely expressed, with highest levels in adult muscle, kidney, lung, liver, and fetal muscle.

Experimental Guidance and Technical Tips

• For Western blotting, the predicted molecular weight of NRF2 is approximately 68 kDa; consider using positive control lysates from tissues with high endogenous expression such as liver, kidney, or muscle.
• Because NRF2 is regulated by oxidative stress and autophagy, induction or stress conditions may be necessary to observe nuclear translocation; validate in the relevant sample system.
• In immunohistochemistry (IHC-P), appropriate antigen retrieval and permeabilization steps may be required to detect both cytoplasmic and nuclear NRF2.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to validated antibody pairs, optimized polyclonal antibodies, bulk ancillary reagents, and rare target sourcing for oxidative stress and cytoprotection research. Our NRF2 rabbit polyclonal antibody is part of a comprehensive portfolio designed to accelerate your IVD assay development from concept to clinic, with reliable support for WB, IHC-P, and ELISA applications.