Anti-NRF1 Rabbit Polyclonal Antibody for WB, ELISA and ChIP - Q16656

Core Product Specifications and Parameters

Biological Background: NRF1 Function and Localization

• Nuclear respiratory factor 1 (NRF1), also known as Alpha palindromic-binding protein (Alpha-pal), is a transcription factor encoded by the NRF1 gene in humans.
• NRF1 activates the expression of EIF2S1 (EIF2-alpha), linking transcriptional modulation of key metabolic genes to cellular growth and development.
• It controls nuclear genes required for mitochondrial respiration, heme biosynthesis, and mitochondrial DNA transcription and replication.
• The protein localizes to the nucleus.
• NRF1 is ubiquitously expressed, with the highest levels observed in skeletal muscle.
• The protein undergoes alternative splicing and is subject to post-translational modifications including phosphorylation and ubiquitination, as indicated by keywords (Phosphoprotein, Ubl conjugation, Isopeptide bond).
• Structural studies (3D-structure keyword) have provided insights into its DNA-binding domain.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant protein corresponding to amino acids 240-449 of human NRF1, which may be considered for detecting specific isoforms or splice variants.
• Validated applications include Western blot (WB), ELISA, and chromatin immunoprecipitation (ChIP); it is advisable to optimize dilution and conditions for each assay.
• Cross-reactivity is reported with human, mouse, and rat samples; confirm reactivity in your specific experimental system.
• Since this is a rabbit polyclonal antibody, batch-to-batch consistency should be verified for quantitative studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, technical services, and consulting—from concept to clinic. This anti-NRF1 antibody supports research in transcriptional regulation and mitochondrial biology. We supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to streamline your diagnostic development.