Anti-NRD1 Rabbit Polyclonal Antibody for WB, ELISA - O43847

Core Product Specifications and Parameters

Biological Background: NRD1 (Nardilysin) Function and Localization

• NRD1, also known as nardilysin or N-arginine dibasic convertase (NRD-C), is a metalloprotease that cleaves peptide substrates at the N-terminus of arginine residues in dibasic pairs.
• It acts as a critical activator of BACE1- and ADAM17-mediated pro-neuregulin ectodomain shedding, which is involved in positive regulation of axonal maturation and myelination.
• NRD1 is required for proper functioning of the 2-oxoglutarate dehydrogenase (OGDH) complex, a key enzyme in the citric acid cycle.
• The protein localizes to mitochondria and to cell projections such as dendrites, consistent with roles in energy metabolism and neuronal function.
• It is predominantly expressed in adult heart, skeletal muscle, and testis, with lower levels in other tissues.
• NRD1 is classified as a zinc-dependent metalloprotease and undergoes alternative splicing and phosphorylation.
• The UniProt record includes keywords such as Hydrolase, Metal-binding, Metalloprotease, Mitochondrion, Phosphoprotein, Protease, Signal, and Zinc.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 160–400 of human NRD1; verify that the antibody recognizes your isoform of interest.
• For Western blot, a band near 132 kDa is expected; high-molecular-weight proteins may require extended transfer times or low-percentage gels.
• The antibody is reported to cross-react with human, mouse, and rat; for other species, check sequence homology before use.
• When establishing an ELISA, consider pairing this antibody with an appropriate capture or detection reagent and perform titration experiments.
• Non-specific binding can be minimized by optimizing blocking conditions and antibody dilution.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, supporting diagnostic manufacturers and researchers from concept to clinic. This anti-NRD1 polyclonal antibody is available alongside a broad range of validated antibody pairs, monoclonals, bulk ancillary reagents, and custom sourcing for rare targets. Our portfolio covers protease and neuroscience research areas, ensuring reliable supply for your assay development and manufacturing needs.