Anti-NRCAM Polyclonal Antibody for WB and ELISA - Q92823

Core Product Specifications and Parameters

Biological Background: NRCAM Function and Localization

• NRCAM (Neuronal cell adhesion molecule) is a cell adhesion protein of the immunoglobulin superfamily that mediates cell-cell interactions in the nervous system.
• It plays a critical role in neurite outgrowth in response to contactin binding, contributing to neuronal development and regeneration.
• NRCAM facilitates cell-cell contacts between Schwann cells and axons, essential for proper myelination and peripheral nerve function.
• The protein is required for the formation and maintenance of nodes of Ranvier, where it organizes sodium channel clustering to enable saltatory conduction.
• NRCAM localizes to the cell membrane, axon projections, and can be secreted, reflecting its roles in both cell-surface adhesion and extracellular signaling.
• It is broadly expressed, with detection in brain regions such as the amygdala, hippocampus, and thalamus, as well as in non-neural tissues.
• Post-translational modifications include glycosylation, disulfide bond formation, and phosphorylation; alternative splicing produces multiple isoforms.

Experimental Guidance and Technical Tips

• For western blotting, use reducing conditions and note the predicted molecular weight of approximately 144 kDa; include appropriate loading controls.
• Since NRCAM can be secreted, consider analyzing both whole-cell lysates and conditioned medium to capture all forms.
• For ELISA, validate the antibody with the recombinant immunogen or a characterized protein standard to ensure specificity.
• Confirm cross-reactivity in your specific sample system, as reactivity is reported for human and mouse.
• Optimize primary antibody dilution and blocking conditions empirically for your assay format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, including antibodies for neurobiology targets such as this anti-NRCAM polyclonal antibody. We streamline procurement of validated antibody pairs, bulk ancillary reagents, and custom raw-material sourcing, helping you accelerate assay development in neuronal disease research. From concept to clinical application, CamelBio is your dedicated partner in building reliable diagnostic solutions.