Anti-NR5A2 Polyclonal Antibody for WB, IHC-P - O00482

Core Product Specifications and Parameters

Biological Background: NR5A2 Function and Localization

• NR5A2 (nuclear receptor subfamily 5 group A member 2), also known as LRH-1, FTF, or CPF, is an orphan nuclear receptor that regulates gene expression by binding as a monomer to specific DNA sequences. It contains zinc-finger domains essential for DNA recognition and transcriptional activation.
• NR5A2 controls key biological processes, including cholesterol and bile acid synthesis pathways, as well as liver and pancreas morphogenesis. Related references: PMID:16289203 PMID:18410128 PMID:21614002
• As a pioneer transcription factor, NR5A2 can induce local chromatin opening by unwrapping target DNA from histones, facilitating gene activation. Related references: PMID:38409506
• NR5A2 plays a critical role in early embryonic development by maintaining epiblast pluripotency and upregulating POU5F1/OCT4 expression. Related references: PMID:27984042
• Although its endogenous ligand remains unknown, specific phospholipids can bind NR5A2 in vitro, promoting a conformational change that recruits coactivators. Related references: PMID:15707893 PMID:15723037 PMID:15897460
• A key transcriptional regulator of lipid metabolism, NR5A2 directly activates the cholesterol 7-alpha-hydroxylase (CYP7A) gene in liver. Related references: PMID:10359768 PMID:20159957
• NR5A2 exhibits anti-inflammatory functions; in the liver, it acts as a corepressor to inhibit the acute phase response. Related references: PMID:20159957
• During hepatitis B virus infection, NR5A2 cooperates with HNF1A to enhance viral gene transcription and DNA replication via the enhancer II element.
• NR5A2 localizes predominantly to the nucleus and associates with chromatin. It is highly expressed in pancreas, with lower levels in liver, and is detected in HepG2 cells.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 300–541 within the ligand-binding domain; epitope accessibility may vary depending on fixation and denaturation conditions.
• When performing Western blotting, the predicted molecular weight is 61 kDa; potential post‑translational modifications (e.g., ubiquitination, sumoylation) may produce additional bands or slight shifts.
• For IHC‑P and IF‑P, consider optimizing antigen retrieval (e.g., heat‑induced epitope retrieval) and permeabilization steps suitable for nuclear targets.
• Validate the antibody in your specific tissue context—NR5A2 expression is abundant in pancreas but lower in other tissues such as liver, heart, or lung.
• Given cross‑reactivity with mouse and rat, this antibody is suitable for rodent models; confirm working dilutions empirically for each application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research teams with a comprehensive portfolio of IVD raw materials, from validated antibody pairs and monoclonal/polyclonal antibodies to bulk ancillary reagents. The NR5A2 polyclonal antibody supports research into liver function, metabolic disease, and viral hepatitis—areas where consistent reagent supply is critical for assay development. For rare target antigens or customized bulk orders, our scientific team offers tailored sourcing solutions to accelerate your path from concept to clinic.