Anti-NR2F2 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P24468

Core Product Specifications and Parameters

Biological Background: NR2F2 Function and Localization

• NR2F2 (also known as COUP-TF II, ARP-1, COUPTF2) is a nuclear receptor protein that functions as a ligand-activated transcription factor.

• It regulates transcription by binding to specific DNA sites, such as DNA site A, and is involved in controlling the expression of apolipoprotein A-I.

• The protein contains zinc-finger motifs, characteristic of nuclear hormone receptors, enabling DNA-binding and transcriptional regulation.

• NR2F2 is activated by high concentrations of 9-cis-retinoic acid and all-trans-retinoic acid in vitro, but not by dexamethasone, cortisol, or progesterone.

• Subcellular localization is predominantly nuclear, reflecting its role in gene expression control.

• Post-translational modifications include phosphorylation, as indicated by proteomics identification.

• Tissue expression is ubiquitous; notably, NR2F2 is expressed in the stromal cells of developing fetal ovaries and may be required to establish ovary identity during early gonad development (PubMed:29478779). Related references: PMID:29478779

• Alternative splicing generates multiple isoforms, and mutations in NR2F2 have been associated with disease variants.

Experimental Guidance and Technical Tips

• The immunogen was designed against the N-terminal region of human NR2F2 (residues 1–100), a domain that may offer target specificity; consider sequence homology when assessing cross-reactivity.

• Based on the supplier's testing, the antibody is qualified for WB, IHC-P, IF/ICC, and ELISA; however, optimal dilutions and conditions should be determined experimentally for your specific samples.

• Reported cross-reactivity with mouse and rat NR2F2 suggests potential utility across these commonly used model organisms; sequence alignment with your species of interest is recommended.

• For IHC-P, anticipate nuclear staining consistent with NR2F2's localization; antigen retrieval and detection methods may need optimization to achieve clear signals.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with streamlined access to validated antibody pairs, optimized polyclonal antibodies like this anti-NR2F2 product, and ancillary reagents to support your IVD development. For projects involving transcription factor analysis and gonad development research, our integrated supply capabilities help bridge raw-material sourcing gaps from concept to clinic. Contact us to explore how we can fulfill your specific bulk and rare-target needs.