Anti-NR2F1 Rabbit Polyclonal Antibody for IHC-P, ELISA - P10589

Core Product Specifications and Parameters

Biological Background: NR2F1 Function and Localization

• NR2F1 encodes COUP transcription factor 1 (COUP-TF I), a member of the nuclear receptor superfamily that functions as a transcriptional regulator.
• COUP-TF I binds to the ovalbumin promoter and, together with the cofactor S300-II, stimulates transcription initiation.
• It recognizes both direct repeats and palindromic sequences of the 5'-AGGTCA-3' motif, allowing versatile DNA-binding.
• Acts as a transcriptional repressor of the luteinizing hormone/choriogonadotropin receptor (LHCG) gene, highlighting its role in hormonal regulation.
• The protein contains zinc finger motifs and belongs to a class of zinc-binding transcription factors.
• Subcellularly localized to the nucleus, consistent with its role in gene expression regulation.
• Alternative names include EAR-3 (v-erbA-related protein 3) and NR2F1, reflecting its homology to the oncogene v-erbA.
• It is involved in various biological processes and has been associated with disease variants when mutated.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 60-180 of human NR2F1; this region may be conserved across species—verify species cross-reactivity if needed.
• For IHC-P, consider using antigen retrieval methods and validate with both positive and negative human tissue samples.
• In ELISA, pair this antibody with an appropriate detection system; consider its polyclonal nature for potential non-specific binding.
• As a rabbit polyclonal antibody, it may be used for immunoprecipitation or other applications after end-user optimization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting. For your NR2F1-related research and assay development, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. Our rare target raw-material sourcing capabilities ensure you can move from concept to clinic efficiently.