Anti-NR2E1/TLX Polyclonal Antibody for WB, ELISA - Q9Y466

Core Product Specifications and Parameters

Biological Background: NR2E1/TLX Function and Localization

• NR2E1 (nuclear receptor subfamily 2 group E member 1), also known as nuclear receptor TLX or protein tailless homolog, is an orphan nuclear receptor encoded by the NR2E1 (TLX) gene.
• It functions as a DNA-binding transcription factor that recognizes hormone response elements (HRE) with the extended core motif 5'-AAGGTCA-3', with flanking nucleotides contributing to receptor specificity (By similarity).
• NR2E1 is critical for embryonic brain development, potentially patterning anterior brain differentiation, and shows highest expression in adult brain regions such as the caudate nucleus and hippocampus.
• It is essential for retinal development and vision; during retinogenesis, NR2E1 can suppress PTEN transcription by binding its promoter, thereby modulating Cyclin D expression and cell cycle progression (By similarity).
• The protein may also be involved in retinoic acid receptor (RAR) regulation in retinal cells, linking it to retinoid signaling pathways.
• As an orphan receptor, NR2E1 can act as both a transcriptional activator and repressor, depending on cellular context.
• It localizes predominantly to the nucleus, consistent with its role as a transcription factor.
• Structurally, NR2E1 contains zinc-finger domains and undergoes alternative splicing, with a calculated molecular weight of approximately 43 kDa.

Experimental Guidance and Technical Tips

• The immunogen spans residues 106–385 of human NR2E1, which includes part of the DNA-binding domain and ligand-binding domain, suggesting the polyclonal antibody may recognize multiple epitopes.
• For Western blot, expect a band around 43 kDa in cell or tissue lysates expressing NR2E1. Optimal loading and antibody dilution should be determined empirically using positive controls such as brain or retinal tissue.
• This antibody has been shown to react with both human and mouse NR2E1 by ELISA and WB; cross-reactivity with other species should be verified if needed.
• For ELISA applications, consider using recombinant NR2E1 protein (e.g., the immunogen) as a standard and coat antigen, and include appropriate negative controls to assess specificity.
• Since NR2E1 is a transcription factor, detection may be enhanced by using nuclear-enriched lysates. Post-translational modifications (e.g., phosphorylation) could potentially affect antibody binding; evaluate under relevant experimental conditions.

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