Anti-NR2E1/TLX Monoclonal Antibody for WB and ELISA - Q9Y466

Core Product Specifications and Parameters

Biological Background: NR2E1/TLX Function and Localization

• NR2E1 (Nuclear receptor subfamily 2 group E member 1), also known as TLX or tailless homolog, is an orphan nuclear receptor and transcription factor.
• It binds DNA as a monomer to hormone response elements with a core motif half-site 5'-AAGGTCA-3', acting as an activator or repressor depending on context.
• NR2E1 is essential for anterior brain differentiation, with highest expression in the caudate nucleus and hippocampus, and lower levels in the thalamus.
• In retinal development, NR2E1 regulates PTEN and Cyclin D expression by binding to the PTEN promoter and suppressing its activity, playing a crucial role in vision.
• The protein localizes exclusively to the nucleus and contains zinc-finger domains, characteristic of DNA-binding nuclear receptors.
• Alternative splicing generates multiple isoforms, and the receptor may interact with retinoic acid receptor (RAR) pathways in retinal cells.

Experimental Guidance and Technical Tips

• For Western blot, use brain tissue lysates (e.g., from hippocampus or caudate nucleus) as positive controls to confirm expression of the 43kDa NR2E1 protein.
• Since the immunogen is a synthetic peptide from the N-terminus (aa1-100), this antibody may recognize multiple isoforms if the epitope is conserved; validate in your relevant sample system.
• For ELISA, consider coating the antibody directly and using peptide or recombinant protein as standard to generate a calibration curve.
• Cross-reactivity with mouse and rat is expected based on sequence similarity; confirm with species-specific controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research institutes streamlined access to high-quality IVD raw materials, including this rabbit monoclonal NR2E1/TLX antibody. Our one-stop platform covers all stages from concept to clinic, supplying validated antibodies, optimized antibody pairs, and ancillary reagents. Whether you need bulk quantities for kit development or rare-target sourcing for neuroscience and retinal disease markers, we facilitate reliable procurement and technical consulting.