Anti-NR2C2/TR4 Rabbit Monoclonal Antibody for WB and ELISA - P49116

Core Product Specifications and Parameters

Biological Background: Nuclear Receptor Subfamily 2 Group C Member 2 (NR2C2/TR4) Function and Localization

• NR2C2, also known as TR4 or TAK1, is an orphan nuclear receptor encoded by the NR2C2 gene, with alternative splicing producing multiple isoforms.
• It functions as a transcriptional repressor or activator, binding to hormone response elements (HREs) consisting of direct repeat AGGTCA half-sites.
• NR2C2 plays a critical role in repressing nuclear receptor pathways, including retinoic acid, retinoid X, vitamin D3, thyroid hormone, and estrogen receptor signaling.
• Together with NR2C1, it forms the DRED complex that represses embryonic and fetal globin transcription, including GATA1.
• The protein is essential for early embryonic development, embryonic stem cell maintenance, spermatogenesis, and cerebellum development.
• NR2C2 is localized primarily to the nucleus, consistent with its function as a transcription factor.
• Post-translational modifications include acetylation, phosphorylation, isopeptide bond formation, and ubiquitin conjugation, which likely regulate its activity and stability.
• It contains zinc-finger motifs of the nuclear receptor type, enabling DNA binding and receptor function.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal region (aa 460-555) of human NR2C2; consider sequence alignment to verify target specificity across isoforms and species.
• For Western blot, the predicted molecular weight is ~65 kDa; validate the band size in your sample system, as post-translational modifications or isoforms may cause shifts.
• In ELISA, the antibody can be used for direct or sandwich formats; optimization of coating and detection conditions is recommended.
• Cross-reactivity is reported for human, mouse, and rat; for other species, check sequence homology before use.
• Use appropriate nuclear protein extraction methods to enrich the target protein from cell or tissue lysates.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-NR2C2/TR4 antibody supports research into nuclear receptor signaling pathways, spermatogenesis, and embryonic development. Beyond this clone, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target raw-material sourcing to streamline your assay development and scale-up.