Anti-NR2C2AP Polyclonal Antibody for WB, ELISA - Q86WQ0

Core Product Specifications and Parameters

Biological Background: NR2C2AP Function and Localization

• NR2C2AP, also known as TRA16, is a 16 kDa nuclear protein that may act as a repressor of NR2C2 (TR4)-mediated transactivation.
• It suppresses the binding between NR2C2/TR4 and the TR4-response element in target genes, thereby modulating gene expression.
• The protein localizes to the nucleus, consistent with its role in transcriptional regulation.
• NR2C2AP is expressed in all tissues examined, with highest expression in heart, skeletal muscle, and pancreas.
• Alternative splicing of the NR2C2AP gene produces multiple transcript variants.
• Keywords from UniProt include Alternative splicing, Nucleus, Proteomics identification, and Reference proteome.

Experimental Guidance and Technical Tips

• For Western blot, the calculated molecular weight of NR2C2AP is 16 kDa; consider using a 12-15% SDS-PAGE gel for optimal separation and validate the signal in human cell lines or tissues with high expression (e.g., heart, skeletal muscle).
• For ELISA, this antibody can detect recombinant NR2C2AP protein. Include appropriate controls and consider testing with human serum or lysates if detecting endogenous protein.
• Since the immunogen covers the N-terminal region (aa 1-139), the antibody may recognize multiple isoforms if alternative splicing retains this region.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—from concept to clinic. Our portfolio includes validated antibody pairs, optimized polyclonal antibodies like this anti-NR2C2AP, and bulk ancillary reagents for gene regulation studies. For hard-to-source targets, we offer custom sourcing solutions to accelerate your assay development.