Anti-NR2C2 Rabbit Polyclonal Antibody for WB, ELISA - P49116

Core Product Specifications and Parameters

Biological Background: NR2C2 Function and Localization

• NR2C2, also known as TR4 or TAK1, is an orphan nuclear receptor that functions as a transcriptional repressor or activator. It belongs to the nuclear receptor subfamily 2 group C.
• It represses signaling pathways of retinoic acid receptor, retinoid X, vitamin D3 receptor, thyroid hormone receptor, and estrogen receptor, and acts as an antagonist of PPARA-mediated transactivation.
• Together with NR2C1, it forms the DRED complex that represses embryonic and fetal globin transcription, including GATA1, by binding to direct repeat hormone response elements (AGGTCA half-sites).
• NR2C2 plays a fundamental role in early embryonic development and embryonic stem cell maintenance, and is required for normal spermatogenesis and cerebellum development.
• It localizes to the nucleus and binds DNA via its zinc-finger domains; it is subject to post-translational modifications including acetylation, phosphorylation, and ubiquitination.
• The protein activates the transcriptional activity of LHCG and is important for neurodevelopmentally regulated behavior (by similarity).

Experimental Guidance and Technical Tips

• For Western blotting, use standard conditions and verify the expected band at approximately 65 kDa in human, mouse, or rat samples. Pre-stained markers are recommended.
• Consider that the immunogen corresponds to amino acids 240–380, which covers a region of NR2C2; antibody reactivity may be affected by post-translational modifications or protein interactions.
• In ELISA applications, the antibody may be used as a capture or detection reagent; optimal coating conditions and detection systems should be empirically determined.
• Since this is a polyclonal antibody, lot-to-lot consistency should be verified in the target assay system; cross-adsorption steps may be required for high-specificity applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with streamlined access to IVD raw materials, technical services, and consulting—spanning concept to clinic. This anti-NR2C2 antibody is positioned for researchers studying transcriptional regulation, nuclear receptor signaling, and reproductive or developmental biology. Our supply network includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to support your assay development and production needs.