Anti-NR2C1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P13056

Core Product Specifications and Parameters

Biological Background: NR2C1 Function and Localization

• NR2C1 is an orphan nuclear receptor, originally designated testicular receptor 2 (TR2), primarily functioning as a transcriptional repressor.
• It binds to the IR7 element in its own gene promoter, establishing an autoregulatory negative feedback loop.
• Together with the closely related NR2C2, it forms the core of the DRED (direct repeat erythroid-definitive) complex that represses embryonic and fetal globin gene transcription.
• In certain contexts, NR2C1 can act as a transcriptional activator, upregulating OCT4 expression, which may facilitate stem cell proliferation and differentiation.
• It mediates retinoic acid-regulated preadipocyte proliferation, implicating it in adipogenesis.
• The protein localizes to the nucleus, including PML nuclear bodies, and contains conserved zinc-finger DNA-binding domains.
• Post-translational modifications, including phosphorylation and ubiquitin conjugation, regulate its stability and transcriptional activity.
• Keywords associated with NR2C1 include activator, repressor, DNA-binding, metal-binding (zinc), phosphoprotein, and Ubl conjugation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1-240, covering the N-terminal region, which may include activation/repression domains; reactivity to isoforms or splice variants should be evaluated.
• For western blot, the predicted molecular weight is ~67 kDa; use appropriate positive and negative controls to confirm specificity in your sample system.
• In immunofluorescence or immunocytochemistry, expect nuclear staining, with potential enrichment in PML bodies; validate with organelle markers or knockout controls.
• This rabbit polyclonal antibody is applicable to ELISA; consider optimizing coating and detection conditions for your assay format.
• Cross-reactivity is reported for human and mouse; for other species, verify sequence homology to the immunogen region.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement by offering a comprehensive portfolio including validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. For transcription regulation and stem cell research involving NR2C1, CamelBio ensures reliable access to high-quality antibodies and can assist in sourcing rare target raw materials to support your diagnostic development from concept to clinic.