Anti-NR1I3 Rabbit Polyclonal Antibody for WB - Q14994

Core Product Specifications and Parameters

Biological Background: NR1I3 Function and Localization

• NR1I3 encodes the constitutive androstane receptor (CAR), a member of the nuclear receptor superfamily; it is also known as MB67 and constitutive activator of retinoid response.
• CAR functions as a ligand-activated transcription factor that binds to retinoic acid response elements (RAREs), thereby controlling expression of genes such as retinoic acid receptor beta 2 and alcohol dehydrogenase 3.
• NR1I3 transactivates the phenobarbital-responsive enhancer module of the human CYP2B6 gene and the CYP3A4 xenobiotic response element, playing a central role in drug metabolism and detoxification.
• The receptor contains a DNA-binding domain with two zinc-finger motifs and a ligand-binding domain, consistent with its zinc-binding and DNA-binding activities.
• Subcellularly, CAR localizes predominantly in the nucleoplasm but is also found in the cytoplasm and associated with cytoskeletal elements, reflecting dynamic intracellular trafficking.
• Expression of NR1I3 is predominantly hepatic, with highest mRNA levels detected in liver tissue.
• Post-translational modifications, including phosphorylation, regulate CAR activity and subcellular localization, impacting its transcriptional function.
• Alternative splicing generates multiple transcript variants, potentially diversifying the functional repertoire of NR1I3.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 103-352 of human NR1I3, spanning the hinge and ligand-binding domains; this region is relatively conserved, supporting expected cross-reactivity with mouse and rat orthologs.
• For Western blotting, a band approximately 40 kDa is anticipated; consider using whole-cell lysates from liver tissue or hepatoma cell lines (e.g., HepG2) to validate endogenous expression.
• In immunofluorescence/ICC, NR1I3 may exhibit both nuclear and cytoplasmic staining; fixation and permeabilization protocols should be optimized to preserve cytoskeletal-associated pools.
• This rabbit polyclonal antibody is suitable for immunoprecipitation (IP), but binding efficiency to native CAR complexes should be empirically determined in the relevant cellular context.
• ELISA applications may benefit from using recombinant NR1I3 protein or peptide as capture antigen; dilution and detection conditions require end-user optimization.

CamelBio: Your One-Stop Sourcing Bridge

This anti-NR1I3 polyclonal antibody is part of CamelBio's comprehensive portfolio for nuclear receptor and drug metabolism research. As your one-stop IVD raw-material partner, CamelBio offers validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets—supporting diagnostic manufacturers and research institutes from concept to clinic with reliable supply solutions.