Anti-NR1H4 Rabbit Polyclonal Antibody for WB and ELISA - Q96RI1

Core Product Specifications and Parameters

Biological Background: NR1H4 Function and Localization

• NR1H4 (also known as FXR) is a ligand-activated nuclear receptor and primary sensor for bile acids such as chenodeoxycholic acid; it plays an essential role in maintaining bile acid, lipid, and glucose homeostasis. Related references: PMID:10334992 PMID:10334993 PMID:21383957
• NR1H4 directly regulates genes critical for bile acid synthesis, conjugation, and transport, including indirect repression of CYP7A1 via NR0B2, activation of the bile salt export pump ABCB11/BSEP, and induction of intestinal FGF19 to provide feedback inhibition of hepatic bile acid synthesis. Related references: PMID:12754200 PMID:15471871 PMID:17895379 PMID:10514450 PMID:15239098 PMID:16269519 PMID:12815072 PMID:19085950 PMID:12806625 PMID:16946559
• Beyond bile acid metabolism, NR1H4 modulates lipid homeostasis by regulating genes involved in lipoprotein metabolism and triglyceride clearance, and it influences glucose homeostasis through effects on hepatic gluconeogenesis and insulin secretion. Related references: PMID:12554753 PMID:12660231 PMID:15337761 PMID:11579204 PMID:11927623 PMID:21804189 PMID:12891557 PMID:20447400
• NR1H4 exerts anti-inflammatory effects in the liver and intestine, down-regulating pro-inflammatory cytokine expression and mediating trans-repression of TLR4 signaling; this activity involves sumoylation and interaction with the N-CoR corepressor. Related references: PMID:21242261 PMID:19864602
• NR1H4 is predominantly localized to the nucleus and is expressed in multiple tissues: liver and hepatocyte-related cells express mainly FXRalpha1-type isoforms (isoforms 3 and 4), while intestine and kidney express FXRalpha2-type isoforms (isoforms 1 and 2); it is also present in pancreatic beta cells and macrophages.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein comprising amino acids 1–230 of human NR1H4 (NP_001193922.1), covering the N-terminal region and possibly the DNA-binding domain; consider epitope accessibility when planning experiments.
• Applications validated by the manufacturer include Western blotting (WB) and ELISA; for WB, a band at ~56 kDa is expected, but verification in the relevant sample system is advised.
• Reported cross-reactivity with mouse and rat suggests utility in rodent models, but researchers should validate reactivity in their specific experimental context.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories streamlined access to a comprehensive portfolio of IVD raw materials, including validated polyclonal and monoclonal antibodies, antibody pairs, and ancillary reagents. For nuclear receptor targets such as NR1H4—essential in metabolic and liver disease research—CamelBio can assist in sourcing bulk quantities and rare antibody preparations to support assay development and production. Our one-stop service integrates technical consultation and supply chain management to accelerate your diagnostic project.