Anti-NR0B1 Polyclonal Antibody for WB, IF/ICC, ELISA - P51843

Core Product Specifications and Parameters

Biological Background: NR0B1 Function and Localization

• Encodes nuclear receptor DAX-1 (NR0B1), an atypical nuclear receptor that lacks a DNA-binding domain.
• Functions as a corepressor that inhibits the transcriptional activity of other nuclear receptors through heterodimeric interactions. Related references: PMID:12482977 PMID:32433991
• Plays a critical role in the development of the hypothalamic-pituitary-adrenal-gonadal (HPA) axis. Related references: PMID:7990953 PMID:8675564
• May contribute to embryonic development and the maintenance of embryonic stem cell pluripotency (By similarity).
• Localizes to both the nucleus and cytoplasm.
• Undergoes alternative splicing, giving rise to multiple isoforms.
• Associated with human diseases such as X-linked adrenal hypoplasia congenita and dosage-sensitive sex reversal (based on UniProt keyword 'Disease variant').

Experimental Guidance and Technical Tips

• The immunogen used corresponds to the N-terminal region (aa 1-260) of human NR0B1, which encompasses domains potentially involved in protein-protein interactions. For Western blot (WB) and immunofluorescence (IF/ICC), consider validating the antibody in cell lines or tissues known to express NR0B1 (e.g., adrenal gland, reproductive tissues).
• Given the indicated cross-reactivity with mouse, this antibody may be suitable for mouse model studies. Validate in specific mouse tissue extracts or cell lines to confirm performance.
• For ELISA applications, optimize the coating antigen and antibody dilution in the relevant sample matrix.
• As a polyclonal antibody, lot-to-lot consistency should be verified for critical experiments, and appropriate controls (e.g., negative tissue/cell lines) are recommended.

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CamelBio provides diagnostic manufacturers, labs, and research institutes with efficient access to IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. By streamlining supply chains and offering technical support, CamelBio accelerates the development of assays targeting nuclear receptors and endocrine pathway proteins like NR0B1. Partner with CamelBio to secure high-quality raw materials for your next diagnostic or research project.