Anti-NQO2 Polyclonal Antibody for WB, IHC-P, ELISA - P16083

Core Product Specifications and Parameters

Biological Background: NQO2 Function and Localization

• NQO2 (ribosyldihydronicotinamide dehydrogenase [quinone]), also known as NRH:quinone oxidoreductase 2 or quinone reductase 2 (QR2), is a flavoprotein that catalyzes the reduction of quinones to hydroquinones.
• The enzyme serves as a quinone reductase in detoxification pathways, facilitating conjugation reactions of hydroquinones.
• NQO2 participates in the vitamin K-dependent gamma-carboxylation of glutamate residues, playing a role in prothrombin synthesis.
• It is localized to the cytoplasm.
• NQO2 is a zinc-binding metalloprotein and is subject to phosphorylation, suggesting regulatory modifications.
• The protein has a predicted molecular weight of approximately 26 kDa (calculated from sequence 25919 Da).

Experimental Guidance and Technical Tips

• For Western blot, a band around 26 kDa is expected; consider optimizing protein loading and transfer conditions.
• For immunohistochemistry (IHC-P), antigen retrieval methods and antibody dilution should be empirically determined for each tissue type.
• For ELISA, the antibody can be utilized as either a capture or detection reagent; validate performance in the relevant sample system.
• Since the immunogen covers nearly the full-length human NQO2 sequence, the antibody may recognize multiple conformational epitopes; cross-reactivity with mouse is indicated, but other species should be tested.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-NQO2 polyclonal antibody supports research into detoxification pathways and vitamin K-dependent processes. Through our extensive supply network, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents; let us bridge your sourcing needs for rare or niche target raw materials.