Anti-NQO2 Polyclonal Antibody for WB, ELISA - P16083

Core Product Specifications and Parameters

Biological Background: NQO2 Function and Localization

• NQO2, also known as ribosyldihydronicotinamide dehydrogenase [quinone], NRH:quinone oxidoreductase 2, and quinone reductase 2 (QR2), is a cytoplasmic enzyme.
• It functions as a quinone reductase, catalyzing the reduction of quinones to hydroquinones, which is essential for detoxification pathways and biosynthetic processes such as vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis.
• The enzyme uses FAD as a cofactor and contains a zinc binding site, consistent with the UniProt keywords "FAD", "Flavoprotein", "Metal-binding", and "Zinc".
• It is classified as a phosphoprotein, suggesting regulation by phosphorylation events.
• Subcellular localization is primarily cytoplasmic, as indicated by the "Cytoplasm" keyword.
• The calculated molecular weight is approximately 25.9 kDa, which aligns with the observed 26 kDa band in western blotting.

Experimental Guidance and Technical Tips

• The immunogen covers the near full-length NQO2 protein (amino acids 1-231), which may make this antibody suitable for detecting both native and denatured protein forms in WB and ELISA.
• For WB, consider using positive control lysates from tissues with known NQO2 expression, such as human liver or kidney.
• The confirmed cross-reactivity with mouse enables use in mouse models; validate detection in the relevant mouse tissue or cell line.
• Optimize antibody dilution and blocking conditions for each experimental system to ensure specific signal.
• For ELISA, the recombinant immunogen protein can serve as a positive control to establish assay sensitivity and linearity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with streamlined access to high-quality IVD raw materials. This anti-NQO2 antibody is offered alongside our comprehensive portfolio of validated antibody pairs, monoclonal/polyclonal antibodies, and ancillary reagents. Our sourcing expertise ensures reliable supply for quinone reductase and detoxification research applications.