Anti-NQO2 Monoclonal Antibody for WB, ELISA - P16083

Core Product Specifications and Parameters

Biological Background: NQO2 Function and Localization

• NQO2 (also known as QR2, DHQV, DIA6, NMOR2) is a cytoplasmic flavoprotein that functions as an NRH:quinone oxidoreductase, catalyzing two-electron reduction of quinones to hydroquinones.
• It plays a critical role in cellular detoxification pathways by reducing quinones, facilitating their conjugation and elimination, and participates in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis.
• The enzyme requires FAD as a cofactor and binds zinc ions, as indicated by structural studies.
• Post-translational modifications include phosphorylation, which may regulate its activity.
• NQO2 is primarily localized to the cytoplasm, where it exerts its oxidoreductase function.
• The protein is a reference proteome member with a predicted molecular weight of approximately 26 kDa (25919 Da from UniProt; 26 kDa observed by supplier).

Experimental Guidance and Technical Tips

• The antibody was generated using a synthetic peptide immunogen spanning the N-terminal 1–100 amino acids of human NQO2; for applications, consider that epitope accessibility may vary depending on sample preparation and assay conditions.
• For Western blot (WB) analysis, a band corresponding to the protein molecular weight of approximately 26 kDa is expected; validate using appropriate positive controls such as cell lines expressing NQO2.
• This antibody cross-reacts with mouse and rat; when working with these species, perform a pilot experiment to confirm reactivity in your specific tissue or cell lysate.
• ELISA applications may be explored; consider direct or sandwich ELISA formats as per your assay requirements and validate with recombinant NQO2 protein if available.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your comprehensive one-stop sourcing bridge for IVD raw materials, offering validated antibody pairs, monoclonal and polyclonal antibodies, and bulk ancillary reagents. For researchers studying NQO2 in detoxification pathways or vitamin K metabolism, CamelBio provides access to this anti-NQO2 monoclonal antibody along with technical consultation to support your diagnostic development from concept to clinic. Our streamlined supply capabilities help you secure rare-target reagents efficiently, letting you focus on advancing your assays.